3kur

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{{Seed}}
 
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[[Image:3kur.jpg|left|200px]]
 
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==Crystal structure of the MLLE domain of poly(A)-binding protein==
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The line below this paragraph, containing "STRUCTURE_3kur", creates the "Structure Box" on the page.
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<StructureSection load='3kur' size='340' side='right'caption='[[3kur]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kur]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KUR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_3kur| PDB=3kur | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kur OCA], [https://pdbe.org/3kur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kur RCSB], [https://www.ebi.ac.uk/pdbsum/3kur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kur ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PABP1_HUMAN PABP1_HUMAN] Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.<ref>PMID:11051545</ref> <ref>PMID:18447585</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/3kur_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kur ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MLLE (previously known as PABC) is a peptide-binding domain that is found in poly(A)-binding protein (PABP) and EDD (E3 isolated by differential display), a HECT E3 ubiquitin ligase also known as HYD (hyperplastic discs tumor suppressor) or UBR5. The MLLE domain from PABP recruits various regulatory proteins and translation factors to poly(A) mRNAs through binding of a conserved 12 amino acid peptide motif called PAM2 (for PABP-interacting motif 2). Here, we determined crystal structures of the MLLE domain from PABP alone and in complex with PAM2 peptides from PABP-interacting protein 2. The structures provide a detailed view of hydrophobic determinants of the MLLE binding coded by PAM2 positions 3, 5, 7, 10, and 12 and reveal novel intermolecular polar contacts. In particular, the side chain of the invariant MLLE residue K580 forms hydrogen bonds with the backbone of PAM2 residues 5 and 7. The structures also show that peptide residues outside of the conserved PAM2 motif contribute to binding. Altogether, the structures provide a significant advance in understanding the molecular basis for the binding of PABP by PAM2-containing proteins involved in translational control, mRNA deadenylation, and other cellular processes.
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===Crystal structure of the MLLE domain of poly(A)-binding protein===
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Molecular determinants of PAM2 recognition by the MLLE domain of poly(A)-binding protein.,Kozlov G, Menade M, Rosenauer A, Nguyen L, Gehring K J Mol Biol. 2010 Mar 26;397(2):397-407. Epub 2010 Jan 22. PMID:20096703<ref>PMID:20096703</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_20096703}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kur" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20096703 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20096703}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3KUR is a 8 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KUR OCA].
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==Reference==
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<ref group="xtra">PMID:20096703</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Gehring, K.]]
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[[Category: Large Structures]]
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[[Category: Kozlov, G.]]
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[[Category: Gehring K]]
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[[Category: Acetylation]]
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[[Category: Kozlov G]]
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[[Category: All-helical domain]]
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[[Category: Alternative splicing]]
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[[Category: Cytoplasm]]
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[[Category: Methylation]]
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[[Category: Mrna processing]]
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[[Category: Mrna splicing]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Rna binding protein]]
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[[Category: Rna-binding]]
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[[Category: Spliceosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 10 17:10:09 2010''
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Current revision

Crystal structure of the MLLE domain of poly(A)-binding protein

PDB ID 3kur

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