3l25

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{{Seed}}
 
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[[Image:3l25.png|left|200px]]
 
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==Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA==
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The line below this paragraph, containing "STRUCTURE_3l25", creates the "Structure Box" on the page.
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<StructureSection load='3l25' size='340' side='right'caption='[[3l25]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3l25]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L25 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3l25| PDB=3l25 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l25 OCA], [https://pdbe.org/3l25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l25 RCSB], [https://www.ebi.ac.uk/pdbsum/3l25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l25 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ebola viral protein 35 (VP35), encoded by the highly pathogenic Ebola virus, facilitates host immune evasion by antagonizing antiviral signaling pathways, including those initiated by RIG-I-like receptors. Here we report the crystal structure of the Ebola VP35 interferon inhibitory domain (IID) bound to short double-stranded RNA (dsRNA), which together with in vivo results reveals how VP35-dsRNA interactions contribute to immune evasion. Conserved basic residues in VP35 IID recognize the dsRNA backbone, whereas the dsRNA blunt ends are 'end-capped' by a pocket of hydrophobic residues that mimic RIG-I-like receptor recognition of blunt-end dsRNA. Residues critical for RNA binding are also important for interferon inhibition in vivo but not for viral polymerase cofactor function of VP35. These results suggest that simultaneous recognition of dsRNA backbone and blunt ends provides a mechanism by which Ebola VP35 antagonizes host dsRNA sensors and immune responses.
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===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA===
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Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35.,Leung DW, Prins KC, Borek DM, Farahbakhsh M, Tufariello JM, Ramanan P, Nix JC, Helgeson LA, Otwinowski Z, Honzatko RB, Basler CF, Amarasinghe GK Nat Struct Mol Biol. 2010 Feb;17(2):165-72. Epub 2010 Jan 17. PMID:20081868<ref>PMID:20081868</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3l25" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20081868 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20081868}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Ebola virus - Mayinga, Zaire, 1976]]
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3L25 is a 6 chains structure with sequences from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L25 OCA].
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[[Category: Large Structures]]
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[[Category: Amarasinghe GK]]
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==Reference==
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[[Category: Basler CF]]
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<ref group="xtra">PMID:20081868</ref><references group="xtra"/>
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[[Category: Borek DM]]
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[[Category: Zaire ebolavirus]]
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[[Category: Farahbakhsh M]]
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[[Category: Amarasinghe, G K.]]
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[[Category: Helgeson LA]]
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[[Category: Basler, C F.]]
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[[Category: Honzatko RB]]
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[[Category: Borek, D M.]]
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[[Category: Leung DW]]
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[[Category: Farahbakhsh, M.]]
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[[Category: Nix JC]]
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[[Category: Helgeson, L A.]]
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[[Category: Otwinowski Z]]
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[[Category: Honzatko, R B.]]
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[[Category: Prins KC]]
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[[Category: Leung, D W.]]
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[[Category: Ramanan P]]
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[[Category: Nix, J C.]]
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[[Category: Tufariello JM]]
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[[Category: Otwinowski, Z.]]
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[[Category: Prins, K C.]]
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[[Category: Ramanan, P.]]
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[[Category: Tufariello, J M.]]
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[[Category: Coiled coil]]
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[[Category: Host cytoplasm]]
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[[Category: Interferon antiviral system evasion]]
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[[Category: Rna binding domain]]
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[[Category: Rna binding protein]]
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[[Category: Rna binding protein-rna complex]]
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[[Category: Rna replication]]
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[[Category: Rna-binding]]
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[[Category: Transcription]]
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[[Category: Virion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 09:53:30 2010''
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Current revision

Crystal structure of Zaire Ebola VP35 interferon inhibitory domain bound to 8 bp dsRNA

PDB ID 3l25

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