3kng

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{{Seed}}
 
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[[Image:3kng.png|left|200px]]
 
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==Crystal structure of SnoaB, a cofactor-independent oxygenase from Streptomyces nogalater, determined to 1.9 resolution==
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The line below this paragraph, containing "STRUCTURE_3kng", creates the "Structure Box" on the page.
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<StructureSection load='3kng' size='340' side='right'caption='[[3kng]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kng]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KNG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3kng| PDB=3kng | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kng OCA], [https://pdbe.org/3kng PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kng RCSB], [https://www.ebi.ac.uk/pdbsum/3kng PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kng ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNOAB_STRNO SNOAB_STRNO] Involved in the biosynthesis of the anthracycline (aromatic polyketide) antibiotic nogalamycin (PubMed:8760909, PubMed:8668120). Catalyzes the oxygenation of 12-deoxy-nogalonic acid at position 12 to yield nogalonic acid (PubMed:19255477, PubMed:20052967).<ref>PMID:8668120</ref> <ref>PMID:8760909</ref> <ref>PMID:19255477</ref> <ref>PMID:20052967</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/3kng_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kng ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SnoaB is a cofactor-independent monooxygenase that catalyzes the conversion of 12-deoxynogalonic acid to nogalonic acid in the biosynthesis of the aromatic polyketide nogalamycin in Streptomyces nogalater. In vitro (18)O(2) experiments establish that the oxygen atom incorporated into the substrate is derived from molecular oxygen. The crystal structure of the enzyme was determined in two different space groups to 1.7 and 1.9 A resolution, respectively. The enzyme displays the ferredoxin fold, with the characteristic beta-strand exchange at the dimer interface. The crystal structures reveal a putative catalytic triad involving two asparagine residues, Asn18 and Asn63, and a water molecule, which may play important roles in the enzymatic reaction. Site-directed mutagenesis experiments, replacing the two asparagines individually by alanine, led to a 100-fold drop in enzymatic activity. Replacement of an invariant tryptophan residue in the active site of the enzyme by phenylalanine also resulted in an enzyme variant with about 1% residual activity. Taken together, our findings are most consistent with a carbanion mechanism where the deprotonated substrate reacts with molecular oxygen via one electron transfer and formation of a caged radical.
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===Crystal structure of SnoaB, a cofactor-independent oxygenase from Streptomyces nogalater, determined to 1.9 resolution===
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Crystal Structure of the Cofactor-Independent Monooxygenase SnoaB from Streptomyces nogalater: Implications for the Reaction Mechanism.,Grocholski T, Koskiniemi H, Lindqvist Y, Mantsala P, Niemi J, Schneider G Biochemistry. 2010 Jan 12. PMID:20052967<ref>PMID:20052967</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20052967}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kng" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20052967 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20052967}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KNG is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KNG OCA].
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==Reference==
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<ref group="xtra">PMID:20052967</ref><references group="xtra"/>
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[[Category: Streptomyces nogalater]]
[[Category: Streptomyces nogalater]]
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[[Category: Grocholski, T.]]
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[[Category: Grocholski T]]
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[[Category: Koskiniemi, H.]]
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[[Category: Koskiniemi H]]
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[[Category: Lindqvist, Y.]]
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[[Category: Lindqvist Y]]
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[[Category: Mantsala, P.]]
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[[Category: Mantsala P]]
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[[Category: Niemi, J.]]
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[[Category: Niemi J]]
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[[Category: Schneider, G.]]
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[[Category: Schneider G]]
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[[Category: Anthracycline]]
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[[Category: Cofactor-independent]]
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[[Category: Oxidoreductase]]
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[[Category: Oxygenase]]
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[[Category: Polyketide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 09:54:01 2010''
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Current revision

Crystal structure of SnoaB, a cofactor-independent oxygenase from Streptomyces nogalater, determined to 1.9 resolution

PDB ID 3kng

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