3aag

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{{Seed}}
 
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[[Image:3aag.png|left|200px]]
 
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==Crystal structure of C. jejuni pglb C-terminal domain==
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The line below this paragraph, containing "STRUCTURE_3aag", creates the "Structure Box" on the page.
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<StructureSection load='3aag' size='340' side='right'caption='[[3aag]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3aag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_RM1221 Campylobacter jejuni RM1221]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AAG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3aag| PDB=3aag | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aag OCA], [https://pdbe.org/3aag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aag RCSB], [https://www.ebi.ac.uk/pdbsum/3aag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aag ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGLB_CAMJR PGLB_CAMJR] Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins.<ref>PMID:20007322</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/3aag_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3aag ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Oligosaccharyltransferase (OST) catalyzes the transfer of an oligosaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains. In the bacterium Campylobacter jejuni, a single-subunit membrane protein, PglB, catalyzes N-glycosylation. We report the 2.8 A resolution crystal structure of the C-terminal globular domain of PglB and its comparison with the previously determined structure from the archaeon Pyrococcus AglB. The two distantly related oligosaccharyltransferases share unexpected structural similarity beyond that expected from the sequence comparison. The common architecture of the putative catalytic sites revealed a new catalytic motif in PglB. Site-directed mutagenesis analyses confirmed the contribution of this motif to the catalytic function. Bacterial PglB and archaeal AglB constitute a protein family of the catalytic subunit of OST along with STT3 from eukaryotes. A structure-aided multiple sequence alignment of the STT3/PglB/AglB protein family revealed three types of OST catalytic centers. This novel classification will provide a useful framework for understanding the enzymatic properties of the OST enzymes from Eukarya, Archaea, and Bacteria.
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===Crystal structure of C. jejuni pglb C-terminal domain===
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Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases.,Maita N, Nyirenda J, Igura M, Kamishikiryo J, Kohda D J Biol Chem. 2010 Feb 12;285(7):4941-50. Epub 2009 Dec 9. PMID:20007322<ref>PMID:20007322</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20007322}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3aag" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20007322 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20007322}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Campylobacter jejuni RM1221]]
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3AAG is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_rm1221 Campylobacter jejuni rm1221]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AAG OCA].
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[[Category: Large Structures]]
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[[Category: Kohda D]]
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==Reference==
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[[Category: Maita N]]
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<ref group="xtra">PMID:20007322</ref><references group="xtra"/>
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[[Category: Campylobacter jejuni rm1221]]
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[[Category: Kohda, D.]]
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[[Category: Maita, N.]]
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[[Category: Multidomain]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 10:01:32 2010''
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Current revision

Crystal structure of C. jejuni pglb C-terminal domain

PDB ID 3aag

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