2wyy
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2wyy.jpg|left|200px]] | ||
| - | < | + | ==CRYOEM MODEL OF THE VESICULAR STOMATITIS VIRUS== |
| - | + | <SX load='2wyy' size='340' side='right' viewer='molstar' caption='[[2wyy]], [[Resolution|resolution]] 10.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2wyy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus_strain_San_Juan Vesicular stomatitis Indiana virus strain San Juan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WYY FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10Å</td></tr> | |
| - | - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyy OCA], [https://pdbe.org/2wyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wyy RCSB], [https://www.ebi.ac.uk/pdbsum/2wyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyy ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wy/2wyy_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wyy ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and a model system of negative-strand RNA viruses. Through direct visualization by means of cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid. Side-by-side interactions between neighboring N subunits are critical for the nucleocapsid to form a bullet shape, and structure-based mutagenesis results support this description. Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer. | ||
| - | + | Cryo-EM model of the bullet-shaped vesicular stomatitis virus.,Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH Science. 2010 Feb 5;327(5966):689-93. PMID:20133572<ref>PMID:20133572</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2wyy" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| - | + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </SX> | |
| - | == | + | [[Category: Large Structures]] |
| - | + | [[Category: Vesicular stomatitis Indiana virus strain San Juan]] | |
| - | + | [[Category: Ge P]] | |
| - | == | + | [[Category: Green TJ]] |
| - | < | + | [[Category: Luo M]] |
| - | [[Category: Vesicular stomatitis | + | [[Category: Tsao J]] |
| - | [[Category: Ge | + | [[Category: Zhou ZH]] |
| - | [[Category: Green | + | |
| - | [[Category: Luo | + | |
| - | [[Category: Tsao | + | |
| - | [[Category: Zhou | + | |
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Current revision
CRYOEM MODEL OF THE VESICULAR STOMATITIS VIRUS
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