3ioq

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{{Seed}}
 
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[[Image:3ioq.jpg|left|200px]]
 
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==Crystal structure of the Carica candamarcensis cysteine protease CMS1MS2 in complex with E-64.==
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The line below this paragraph, containing "STRUCTURE_3ioq", creates the "Structure Box" on the page.
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<StructureSection load='3ioq' size='340' side='right'caption='[[3ioq]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ioq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vasconcellea_cundinamarcensis Vasconcellea cundinamarcensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IOQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3ioq| PDB=3ioq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ioq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ioq OCA], [https://pdbe.org/3ioq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ioq RCSB], [https://www.ebi.ac.uk/pdbsum/3ioq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ioq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q84XA1_VASCU Q84XA1_VASCU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/3ioq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ioq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cysteine proteinases from the latex of plants of the family Caricaceae are widely used industrially as well as in pharmaceutical preparations. In the present work, a 23 kDa cysteine proteinase from Carica candamarcensis latex (designated CMS1MS2) was purified for crystallization using three chromatography steps. The enzyme shows about fourfold higher activity than papain with BAPNA as substrate. Crystals suitable for X-ray diffraction experiments were obtained by the hanging-drop method in the presence of PEG and ammonium sulfate as precipitants. The crystals are monoclinic (space group P2(1)), with unit-cell parameters a = 53.26, b = 75.71, c = 53.23 A, beta = 96.81 degrees , and diffract X-rays to 1.8 A resolution.
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===Crystal structure of the Carica candamarcensis cysteine protease CMS1MS2 in complex with E-64.===
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Purification, crystallization and preliminary X-ray analysis of CMS1MS2: a cysteine proteinase from Carica candamarcensis latex.,Gomes MT, Teixeira RD, Ribeiro Hde A, Turchetti AP, Junqueira CF, Lopes MT, Salas CE, Nagem RA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jun 1;64(Pt, 6):492-4. Epub 2008 May 16. PMID:018540057<ref>PMID:018540057</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3IOQ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vasconcellea_cundinamarcensis Vasconcellea cundinamarcensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IOQ OCA].
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<div class="pdbe-citations 3ioq" style="background-color:#fffaf0;"></div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:18540057</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vasconcellea cundinamarcensis]]
[[Category: Vasconcellea cundinamarcensis]]
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[[Category: Gomes, M T.R.]]
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[[Category: Gomes MTR]]
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[[Category: Nagem, R A.P.]]
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[[Category: Nagem RAP]]
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[[Category: Salas, C E.]]
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[[Category: Salas CE]]
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[[Category: Teixeira, R D.]]
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[[Category: Teixeira RD]]
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[[Category: Caricaceae]]
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[[Category: Cysteine protease]]
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[[Category: E-64]]
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[[Category: Hydrolase]]
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[[Category: Papain family]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 10:46:10 2010''
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Current revision

Crystal structure of the Carica candamarcensis cysteine protease CMS1MS2 in complex with E-64.

PDB ID 3ioq

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