2wtu

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{{Seed}}
 
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[[Image:2wtu.png|left|200px]]
 
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==Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch.==
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The line below this paragraph, containing "STRUCTURE_2wtu", creates the "Structure Box" on the page.
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<StructureSection load='2wtu' size='340' side='right'caption='[[2wtu]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wtu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WTU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2wtu| PDB=2wtu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wtu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wtu OCA], [https://pdbe.org/2wtu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wtu RCSB], [https://www.ebi.ac.uk/pdbsum/2wtu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wtu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS_ECOLI MUTS_ECOLI] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wt/2wtu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wtu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DNA mismatch repair protein MutS acts as a molecular switch. It toggles between ADP and ATP states and is regulated by mismatched DNA. This is analogous to G-protein switches and the regulation of their "on" and "off" states by guanine exchange factors. Although GDP release in monomeric GTPases is accelerated by guanine exchange factor-induced removal of magnesium from the catalytic site, we found that release of ADP from MutS is not influenced by the metal ion in this manner. Rather, ADP release is induced by the binding of mismatched DNA at the opposite end of the protein, a long-range allosteric response resembling the mechanism of activation of heterotrimeric GTPases. Magnesium influences switching in MutS by inducing faster and tighter ATP binding, allowing rapid downstream responses. MutS mutants with decreased affinity for the metal ion are impaired in fast switching and in vivo mismatch repair. Thus, the G-proteins and MutS conceptually employ the same efficient use of the high energy cofactor: slow hydrolysis in the absence of a signal and fast conversion to the active state when required.
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===CRYSTAL STRUCTURE OF ESCHERICHIA COLI MUTS IN COMPLEX WITH A 16 BASEPAIR OLIGO CONTAINING AN A.A MISMATCH.===
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Magnesium coordination controls the molecular switch function of DNA mismatch repair protein MutS.,Lebbink JH, Fish A, Reumer A, Natrajan G, Winterwerp HH, Sixma TK J Biol Chem. 2010 Apr 23;285(17):13131-41. Epub 2010 Feb 18. PMID:20167596<ref>PMID:20167596</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wtu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20167596}}, adds the Publication Abstract to the page
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20167596 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20167596}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2WTU is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WTU OCA].
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==Reference==
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<ref group="xtra">PMID:20167596</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Fish, A.]]
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[[Category: Large Structures]]
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[[Category: Lebbink, J H.]]
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[[Category: Synthetic construct]]
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[[Category: Natrajan, G.]]
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[[Category: Fish A]]
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[[Category: Reumer, A.]]
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[[Category: Lebbink JH]]
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[[Category: Sixma, T K.]]
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[[Category: Natrajan G]]
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[[Category: Winterwerp, H H.]]
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[[Category: Reumer A]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Sixma TK]]
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[[Category: Dna damage]]
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[[Category: Winterwerp HH]]
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[[Category: Dna repair]]
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[[Category: Nucleotide-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 14:08:50 2010''
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Current revision

Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch.

PDB ID 2wtu

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