3kxp

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{{Seed}}
 
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[[Image:3kxp.png|left|200px]]
 
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==Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase==
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The line below this paragraph, containing "STRUCTURE_3kxp", creates the "Structure Box" on the page.
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<StructureSection load='3kxp' size='340' side='right'caption='[[3kxp]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kxp]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KXP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.26&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3kxp| PDB=3kxp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxp OCA], [https://pdbe.org/3kxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kxp RCSB], [https://www.ebi.ac.uk/pdbsum/3kxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kxp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AAMHY_RHILO AAMHY_RHILO] Catalyzes the final reaction in the degradation of vitamin B6 from (E)-2-(acetamidomethylene)succinate (E-2AMS) to produce succinic semialdehyde, acetate, ammonia and carbon dioxide.<ref>PMID:18635903</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/3kxp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kxp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The gene identification and kinetic characterization of (E)-2-(acetamidomethylene)succinate (E-2AMS) hydrolase has recently been described. This enzyme catalyzes the final reaction in the degradation of vitamin B(6) and produces succinic semialdehyde, acetate, ammonia, and carbon dioxide from E-2AMS. The structure of E-2AMS hydrolase was determined to 2.3 A using SAD phasing. E-2AMS hydrolase is a member of the alpha/beta hydrolase superfamily and utilizes a serine/histidine/aspartic acid catalytic triad. Mutation of either the nucleophilic serine or the aspartate resulted in inactive enzyme. Mutation of an additional serine residue in the active site causes the enzyme to be unstable and is likely structurally important. The structure also provides insight into the mechanism of hydrolysis of E-2AMS and identifies several potential catalytically important residues.
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===Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase===
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Structure Determination and Characterization of the Vitamin B(6) Degradative Enzyme (E)-2-(Acetamidomethylene)succinate Hydrolase (,).,McCulloch KM, Mukherjee T, Begley TP, Ealick SE Biochemistry. 2010 Feb 16;49(6):1226-35. PMID:20099871<ref>PMID:20099871</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20099871}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kxp" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20099871 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20099871}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KXP is a 12 chains structure with sequences from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXP OCA].
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==Reference==
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<ref group="xtra">PMID:20099871</ref><references group="xtra"/>
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[[Category: Mesorhizobium loti]]
[[Category: Mesorhizobium loti]]
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[[Category: Begley, T P.]]
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[[Category: Begley TP]]
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[[Category: Ealick, S E.]]
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[[Category: Ealick SE]]
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[[Category: McCulloch, K M.]]
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[[Category: McCulloch KM]]
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[[Category: Mukherjee, T.]]
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[[Category: Mukherjee T]]
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[[Category: Alpha/beta hydrolase]]
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[[Category: Hydrolase]]
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[[Category: Plp degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 14:55:26 2010''
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Current revision

Crystal Structure of E-2-(Acetamidomethylene)succinate Hydrolase

PDB ID 3kxp

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