2r3a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:44, 30 August 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2r3a.png|left|200px]]
 
-
<!--
+
==Methyltransferase domain of human suppressor of variegation 3-9 homolog 2==
-
The line below this paragraph, containing "STRUCTURE_2r3a", creates the "Structure Box" on the page.
+
<StructureSection load='2r3a' size='340' side='right'caption='[[2r3a]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2r3a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R3A FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_2r3a| PDB=2r3a | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r3a OCA], [https://pdbe.org/2r3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r3a RCSB], [https://www.ebi.ac.uk/pdbsum/2r3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r3a ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SUV92_HUMAN SUV92_HUMAN] Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis.<ref>PMID:14765126</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r3/2r3a_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r3a ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
SET domain methyltransferases deposit methyl marks on specific histone tail lysine residues and play a major role in epigenetic regulation of gene transcription. We solved the structures of the catalytic domains of GLP, G9a, Suv39H2 and PRDM2, four of the eight known human H3K9 methyltransferases in their apo conformation or in complex with the methyl donating cofactor, and peptide substrates. We analyzed the structural determinants for methylation state specificity, and designed a G9a mutant able to tri-methylate H3K9. We show that the I-SET domain acts as a rigid docking platform, while induced-fit of the Post-SET domain is necessary to achieve a catalytically competent conformation. We also propose a model where long-range electrostatics bring enzyme and histone substrate together, while the presence of an arginine upstream of the target lysine is critical for binding and specificity. ENHANCED VERSION: This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1.
-
===Methyltransferase domain of human suppressor of variegation 3-9 homolog 2===
+
Structural biology of human H3K9 methyltransferases.,Wu H, Min J, Lunin VV, Antoshenko T, Dombrovski L, Zeng H, Allali-Hassani A, Campagna-Slater V, Vedadi M, Arrowsmith CH, Plotnikov AN, Schapira M PLoS One. 2010 Jan 11;5(1):e8570. PMID:20084102<ref>PMID:20084102</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2r3a" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_20084102}}, adds the Publication Abstract to the page
+
*[[Histone methyltransferase 3D structures|Histone methyltransferase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 20084102 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_20084102}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2R3A is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R3A OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:20084102</ref><references group="xtra"/>
+
-
[[Category: Histone-lysine N-methyltransferase]]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Arrowsmith, C H.]]
+
[[Category: Large Structures]]
-
[[Category: Bochkarev, A.]]
+
[[Category: Arrowsmith CH]]
-
[[Category: Edwards, A M.]]
+
[[Category: Bochkarev A]]
-
[[Category: Loppnau, P.]]
+
[[Category: Edwards AM]]
-
[[Category: Lunin, V V.]]
+
[[Category: Loppnau P]]
-
[[Category: Min, J.]]
+
[[Category: Lunin VV]]
-
[[Category: Plotnikov, A N.]]
+
[[Category: Min J]]
-
[[Category: Ren, H.]]
+
[[Category: Plotnikov AN]]
-
[[Category: SGC, Structural Genomics Consortium.]]
+
[[Category: Ren H]]
-
[[Category: Sundstrom, M.]]
+
[[Category: Sundstrom M]]
-
[[Category: Weigelt, J.]]
+
[[Category: Weigelt J]]
-
[[Category: Wu, H.]]
+
[[Category: Wu H]]
-
[[Category: Zeng, H.]]
+
[[Category: Zeng H]]
-
[[Category: Alternative splicing]]
+
-
[[Category: Cell cycle]]
+
-
[[Category: Chromatin regulator]]
+
-
[[Category: Chromosomal protein]]
+
-
[[Category: Differentiation]]
+
-
[[Category: H3 lysine-9 specific 2]]
+
-
[[Category: Histone h3-k9 methyltransferase 2]]
+
-
[[Category: Histone-lysine n-methyltransferase]]
+
-
[[Category: Nucleus]]
+
-
[[Category: Repressor]]
+
-
[[Category: S-adenosyl-l-methionine]]
+
-
[[Category: Sgc]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Structural genomics consortium]]
+
-
[[Category: Telomere]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 14:59:24 2010''
+

Current revision

Methyltransferase domain of human suppressor of variegation 3-9 homolog 2

PDB ID 2r3a

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2r3a"
Personal tools