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3ivk
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:3ivk.jpg|left|200px]] | ||
| - | < | + | ==Crystal Structure of the Catalytic Core of an RNA Polymerase Ribozyme Complexed with an Antigen Binding Antibody Fragment== |
| - | + | <StructureSection load='3ivk' size='340' side='right'caption='[[3ivk]], [[Resolution|resolution]] 3.10Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[3ivk]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IVK FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |
| - | -- | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ivk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ivk OCA], [https://pdbe.org/3ivk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ivk RCSB], [https://www.ebi.ac.uk/pdbsum/3ivk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ivk ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/3ivk_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ivk ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Primordial organisms of the putative RNA world would have required polymerase ribozymes able to replicate RNA. Known ribozymes with polymerase activity best approximating that needed for RNA replication contain at their catalytic core the class I RNA ligase, an artificial ribozyme with a catalytic rate among the fastest of known ribozymes. Here we present the 3.0 angstrom crystal structure of this ligase. The architecture resembles a tripod, its three legs converging near the ligation junction. Interacting with this tripod scaffold through a series of 10 minor-groove interactions (including two A-minor triads) is the unpaired segment that contributes to and organizes the active site. A cytosine nucleobase and two backbone phosphates abut the ligation junction; their location suggests a model for catalysis resembling that of proteinaceous polymerases. | ||
| - | + | Crystal structure of the catalytic core of an RNA-polymerase ribozyme.,Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, Bartel DP Science. 2009 Nov 27;326(5957):1271-5. PMID:19965478<ref>PMID:19965478</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3ivk" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| - | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | == | + | [[Category: Large Structures]] |
| - | + | [[Category: Lk3 transgenic mice]] | |
| - | + | [[Category: Bartel, D P]] | |
| - | == | + | [[Category: Duguid, E M]] |
| - | < | + | [[Category: Koide, S]] |
| - | [[Category: | + | [[Category: Koldobskaya, Y]] |
| - | [[Category: Bartel, D P | + | [[Category: Kossiakoff, A A]] |
| - | [[Category: Duguid, E M | + | [[Category: Piccirilli, J A]] |
| - | [[Category: Koide, S | + | [[Category: Shechner, D M]] |
| - | [[Category: Koldobskaya, Y | + | |
| - | [[Category: Kossiakoff, A A | + | |
| - | [[Category: Piccirilli, J A | + | |
| - | [[Category: Shechner, D M | + | |
[[Category: Catalytic rna]] | [[Category: Catalytic rna]] | ||
[[Category: Immune system - rna complex]] | [[Category: Immune system - rna complex]] | ||
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[[Category: Rna hairpin epitope]] | [[Category: Rna hairpin epitope]] | ||
[[Category: Rna polymerase ribozyme]] | [[Category: Rna polymerase ribozyme]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 16:07:47 2010'' | ||
Current revision
Crystal Structure of the Catalytic Core of an RNA Polymerase Ribozyme Complexed with an Antigen Binding Antibody Fragment
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