3hn8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:54, 21 November 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3hn8.png|left|200px]]
 
-
<!--
+
==Crystal structure of synaptotagmin==
-
The line below this paragraph, containing "STRUCTURE_3hn8", creates the "Structure Box" on the page.
+
<StructureSection load='3hn8' size='340' side='right'caption='[[3hn8]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3hn8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HN8 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_3hn8| PDB=3hn8 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hn8 OCA], [https://pdbe.org/3hn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hn8 RCSB], [https://www.ebi.ac.uk/pdbsum/3hn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hn8 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SYT3_RAT SYT3_RAT] May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/3hn8_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hn8 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
In neurons, SNAREs, synaptotagmin and other factors catalyze Ca(2+)-triggered fusion of vesicles with the plasma membrane. The molecular mechanism of this process, especially the interaction between synaptotagmin and SNAREs, remains an enigma. Here we characterized this interaction by single-molecule fluorescence microscopy and crystallography. The two rigid Ca(2+)-binding domains of synaptotagmin 3 (Syt3) undergo large relative motions in solution. Interaction with SNARE complex amplifies a particular state of the two domains that is further enhanced by Ca(2+). This state is represented by the first SNARE-induced Ca(2+)-bound crystal structure of a synaptotagmin fragment containing both domains. The arrangement of the Ca(2+)-binding loops of this structure of Syt3 matches that of SNARE-bound Syt1, suggesting a conserved feature of synaptotagmins. The loops resemble the membrane-interacting loops of certain viral fusion proteins in the postfusion state, suggesting unexpected similarities between both fusion systems.
-
===Crystal structure of synaptotagmin===
+
Molecular mechanism of the synaptotagmin-SNARE interaction in Ca2+-triggered vesicle fusion.,Vrljic M, Strop P, Ernst JA, Sutton RB, Chu S, Brunger AT Nat Struct Mol Biol. 2010 Mar;17(3):325-31. Epub 2010 Feb 21. PMID:20173762<ref>PMID:20173762</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3hn8" style="background-color:#fffaf0;"></div>
-
==About this Structure==
+
==See Also==
-
3HN8 is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HN8 OCA].
+
*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Brunger, A T.]]
+
[[Category: Brunger AT]]
-
[[Category: Ernst, J.]]
+
[[Category: Ernst J]]
-
[[Category: Strop, P.]]
+
[[Category: Strop P]]
-
[[Category: Vrljic, M.]]
+
[[Category: Vrljic M]]
-
[[Category: Calcium]]
+
-
[[Category: Cell junction]]
+
-
[[Category: Cytoplasmic vesicle]]
+
-
[[Category: Membrane]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Signaling protein]]
+
-
[[Category: Synapse]]
+
-
[[Category: Synaptotagmin]]
+
-
[[Category: Transmembrane]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 08:58:19 2010''
+

Current revision

Crystal structure of synaptotagmin

PDB ID 3hn8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hn8"
Personal tools