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3bsy

From Proteopedia

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{{Seed}}
 
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[[Image:3bsy.png|left|200px]]
 
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<!--
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==PglD from Campylobacter jejuni, NCTC 11168, in complex with acetyl coenzyme A==
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The line below this paragraph, containing "STRUCTURE_3bsy", creates the "Structure Box" on the page.
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<StructureSection load='3bsy' size='340' side='right'caption='[[3bsy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bsy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
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{{STRUCTURE_3bsy| PDB=3bsy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsy OCA], [https://pdbe.org/3bsy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsy RCSB], [https://www.ebi.ac.uk/pdbsum/3bsy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGLD_CAMJE PGLD_CAMJE] Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N'-diacetylbacillosamine in the N-linked protein glycosylation pathway.<ref>PMID:17087520</ref> <ref>PMID:19448740</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bsy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bsy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The carbohydrate 2, 4-diacetamido-2, 4, 6-trideoxy-alpha-D-glucopyranose (BacAc(2)) is found in a variety of eubacterial pathogens. In Campylobacter jejuni, PglD acetylates the C4 amino group on UDP-2-acetamido-4-amino-2, 4, 6-trideoxy-alpha-D-glucopyranose (UDP-4-amino-sugar) to form UDP-BacAc(2). Sequence analysis predicts PglD to be a member of the left-handed beta helix family of enzymes. However, poor sequence homology between PglD and left-handed beta helix enzymes with existing structural data precludes unambiguous identification of the active site. The co-crystal structures of PglD in the presence of citrate, acetyl coenzyme A, or the UDP-4-amino-sugar were solved. The biological assembly is a trimer with one active site formed between two protomers. Residues lining the active site were identified, and results from functional assays on alanine mutants suggest His-125 is critical for catalysis, whereas His-15 and His-134 are involved in substrate binding. These results are discussed in the context of implications for proteins homologous to PglD in other pathogens.
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===PglD from Campylobacter jejuni, NCTC 11168, in complex with acetyl coenzyme A===
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Crystal structure and catalytic mechanism of PglD from Campylobacter jejuni.,Olivier NB, Imperiali B J Biol Chem. 2008 Oct 10;283(41):27937-46. Epub 2008 Jul 30. PMID:18667421<ref>PMID:18667421</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18667421}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3bsy" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18667421 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18667421}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3BSY is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSY OCA].
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==Reference==
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<ref group="xtra">PMID:18667421</ref><references group="xtra"/>
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[[Category: Campylobacter jejuni]]
[[Category: Campylobacter jejuni]]
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[[Category: UDP-N-acetylglucosamine diphosphorylase]]
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[[Category: Large Structures]]
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[[Category: Imperiali, B.]]
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[[Category: Imperiali B]]
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[[Category: Olivier, N B.]]
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[[Category: Olivier NB]]
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[[Category: Acetyl coenzyme some]]
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[[Category: Bacillosamine]]
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[[Category: Campylobacter jejuni]]
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[[Category: Hexapeptide repeat]]
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[[Category: Left-hand beta helix]]
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[[Category: N-linked glycosylation]]
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[[Category: Pgl]]
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[[Category: Rossman fold]]
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[[Category: Transferase]]
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[[Category: Udp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 09:04:40 2010''
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Current revision

PglD from Campylobacter jejuni, NCTC 11168, in complex with acetyl coenzyme A

PDB ID 3bsy

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