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1gkp

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[[Image:1gkp.gif|left|200px]]<br />
 
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<applet load="1gkp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gkp, resolution 1.295&Aring;" />
 
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'''D-HYDANTOINASE (DIHYDROPYRIMIDINASE) FROM THERMUS SP. IN SPACE GROUP C2221'''<br />
 
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==Overview==
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==D-Hydantoinase (Dihydropyrimidinase) from Thermus sp. in space group C2221==
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Dihydropyrimidinases (hydantoinases) catalyse the reversible hydrolytic, ring-opening of cyclic diamides such as dihydropyrimidines in the, catabolism of pyrimidines. In biotechnology, these enzymes find, application in the enantiospecific production of amino acids from racemic, hydantoins. The crystal structure of a D-enantio-specific, dihydropyrimidinase from Thermus sp. (D-hydantoinase) was solved de novo, by multiwavelength anomalous diffraction phasing. In spite of a large unit, cell the D-hydantoinase crystals exhibit excellent diffraction properties., The structure was subsequently refined at 1.30 A resolution against native, data. The core of D-hydantoinase consists of a (alpha/beta)(8)-barrel, which is flanked by a beta-sheet domain and some additional helices. In, the active ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12079340 (full description)]]
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<StructureSection load='1gkp' size='340' side='right'caption='[[1gkp]], [[Resolution|resolution]] 1.29&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gkp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_sp. Thermus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GKP FirstGlance]. <br>
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1GKP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermus_sp. Thermus sp.]] with ZN, SO4 and EPE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Dihydropyrimidinase Dihydropyrimidinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.2 3.5.2.2]]. Structure known Active Site: ASA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GKP OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.295&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkp OCA], [https://pdbe.org/1gkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gkp RCSB], [https://www.ebi.ac.uk/pdbsum/1gkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gkp ProSAT]</span></td></tr>
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X-ray structure of a dihydropyrimidinase from Thermus sp. at 1.3 A resolution., Abendroth J, Niefind K, Schomburg D, J Mol Biol. 2002 Jun 28;320(1):143-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12079340 12079340]
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</table>
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[[Category: Dihydropyrimidinase]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q7SIE9_THESP Q7SIE9_THESP]
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[[Category: Thermus sp.]]
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== Evolutionary Conservation ==
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[[Category: Abendroth, J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Niefind, K.]]
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Check<jmol>
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[[Category: Schomburg, D.]]
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<jmolCheckbox>
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[[Category: EPE]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gkp_consurf.spt"</scriptWhenChecked>
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[[Category: SO4]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: ZN]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: cyclic amidase]]
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</jmolCheckbox>
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[[Category: dihydropyrimidinase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gkp ConSurf].
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[[Category: hydantoinase]]
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<div style="clear:both"></div>
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[[Category: hydrolase]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:23:49 2007''
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[[Category: Large Structures]]
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[[Category: Thermus sp]]
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[[Category: Abendroth J]]
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[[Category: Niefind K]]
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[[Category: Schomburg D]]

Current revision

D-Hydantoinase (Dihydropyrimidinase) from Thermus sp. in space group C2221

PDB ID 1gkp

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