3k0s

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{{Seed}}
 
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[[Image:3k0s.png|left|200px]]
 
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==Crystal structure of E.coli DNA mismatch repair protein MutS, D693N mutant, in complex with GT mismatched DNA==
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The line below this paragraph, containing "STRUCTURE_3k0s", creates the "Structure Box" on the page.
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<StructureSection load='3k0s' size='340' side='right'caption='[[3k0s]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3k0s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K0S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K0S FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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{{STRUCTURE_3k0s| PDB=3k0s | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k0s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k0s OCA], [https://pdbe.org/3k0s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k0s RCSB], [https://www.ebi.ac.uk/pdbsum/3k0s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k0s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS_ECOLI MUTS_ECOLI] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/3k0s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k0s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DNA mismatch repair protein MutS acts as a molecular switch. It toggles between ADP and ATP states and is regulated by mismatched DNA. This is analogous to G-protein switches and the regulation of their "on" and "off" states by guanine exchange factors. Although GDP release in monomeric GTPases is accelerated by guanine exchange factor-induced removal of magnesium from the catalytic site, we found that release of ADP from MutS is not influenced by the metal ion in this manner. Rather, ADP release is induced by the binding of mismatched DNA at the opposite end of the protein, a long-range allosteric response resembling the mechanism of activation of heterotrimeric GTPases. Magnesium influences switching in MutS by inducing faster and tighter ATP binding, allowing rapid downstream responses. MutS mutants with decreased affinity for the metal ion are impaired in fast switching and in vivo mismatch repair. Thus, the G-proteins and MutS conceptually employ the same efficient use of the high energy cofactor: slow hydrolysis in the absence of a signal and fast conversion to the active state when required.
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===Crystal structure of E.coli DNA mismatch repair protein MutS, D693N mutant, in complex with GT mismatched DNA===
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Magnesium coordination controls the molecular switch function of DNA mismatch repair protein MutS.,Lebbink JH, Fish A, Reumer A, Natrajan G, Winterwerp HH, Sixma TK J Biol Chem. 2010 Apr 23;285(17):13131-41. Epub 2010 Feb 18. PMID:20167596<ref>PMID:20167596</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3k0s" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20167596}}, adds the Publication Abstract to the page
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*[[ADP-ribose pyrophosphatase 3D structures|ADP-ribose pyrophosphatase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20167596 is the PubMed ID number.
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20167596}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3K0S is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K0S OCA].
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: Reumer GA]]
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<ref group="xtra">PMID:20167596</ref><references group="xtra"/>
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[[Category: Sixma TK]]
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[[Category: Escherichia coli k-12]]
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[[Category: Winterwerp HHK]]
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[[Category: Reumer, G A.]]
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[[Category: Sixma, T K.]]
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[[Category: Winterwerp, H H.K.]]
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[[Category: Atp-binding]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Dna repair protein]]
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[[Category: Dna-binding]]
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[[Category: Magnesium mutant]]
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[[Category: Nucleotide-binding]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 24 08:30:29 2010''
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Current revision

Crystal structure of E.coli DNA mismatch repair protein MutS, D693N mutant, in complex with GT mismatched DNA

PDB ID 3k0s

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