1e9s

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[[Image:1e9s.gif|left|200px]]<br /><applet load="1e9s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e9s, resolution 2.50&Aring;" />
 
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'''BACTERIAL CONJUGATIVE COUPLING PROTEIN TRWBDELTAN70. UNBOUND MONOCLINIC FORM.'''<br />
 
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==Overview==
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==Bacterial conjugative coupling protein TrwBdeltaN70. Unbound monoclinic form.==
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The transfer of DNA across membranes and between cells is a central, biological process; however, its molecular mechanism remains unknown. In, prokaryotes, trans-membrane passage by bacterial conjugation, is the main, route for horizontal gene transfer. It is the means for rapid acquisition, of new genetic information, including antibiotic resistance by pathogens., Trans-kingdom gene transfer from bacteria to plants or fungi and even, bacterial sporulation are special cases of conjugation. An integral, membrane DNA-binding protein, called TrwB in the Escherichia coli R388, conjugative system, is essential for the conjugation process. This large, multimeric protein is responsible for recruiting the relaxosome, DNA-protein complex, and participates in the transfer of a single DNA, strand during cell mating. Here we report the three-dimensional structure, of a soluble variant of TrwB. The molecule consists of two domains: a, nucleotide-binding domain of alpha/beta topology, reminiscent of RecA and, DNA ring helicases, and an all-alpha domain. Six equivalent protein, monomers associate to form an almost spherical quaternary structure that, is strikingly similar to F1-ATPase. A central channel, 20 A in width, traverses the hexamer.
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<StructureSection load='1e9s' size='340' side='right'caption='[[1e9s]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1e9s]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E9S FirstGlance]. <br>
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1E9S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Site: <scene name='pdbsite=NBS:Nucleotide-Binding Site Around Lys136 Of Each Chain'>NBS</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9S OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e9s OCA], [https://pdbe.org/1e9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e9s RCSB], [https://www.ebi.ac.uk/pdbsum/1e9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e9s ProSAT]</span></td></tr>
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==Reference==
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</table>
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The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase., Gomis-Ruth FX, Moncalian G, Perez-Luque R, Gonzalez A, Cabezon E, de la Cruz F, Coll M, Nature. 2001 Feb 1;409(6820):637-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11214325 11214325]
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== Function ==
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[https://www.uniprot.org/uniprot/Q04230_ECOLX Q04230_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e9/1e9s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e9s ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cabezon, E.]]
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[[Category: Cabezon E]]
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[[Category: Coll, M.]]
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[[Category: Coll M]]
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[[Category: Cruz, F.De.La.]]
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[[Category: Gomis-Rueth FX]]
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[[Category: Gomis-Rueth, F.X.]]
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[[Category: Moncalian G]]
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[[Category: Moncalian, G.]]
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[[Category: De la Cruz F]]
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[[Category: bacterial conjugation]]
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[[Category: coupling protein]]
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[[Category: f1-atpase-like quaternary structure]]
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[[Category: ring helicases]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:07:07 2007''
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Current revision

Bacterial conjugative coupling protein TrwBdeltaN70. Unbound monoclinic form.

PDB ID 1e9s

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