3m46

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[[Image:3m46.jpg|left|200px]]
 
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==The crystal structure of the D73A mutant of glycoside HYDROLASE (FAMILY 31) from Ruminococcus obeum ATCC 29174==
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The line below this paragraph, containing "STRUCTURE_3m46", creates the "Structure Box" on the page.
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<StructureSection load='3m46' size='340' side='right'caption='[[3m46]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3m46]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Blautia_obeum_ATCC_29174 Blautia obeum ATCC 29174]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M46 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3m46| PDB=3m46 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m46 OCA], [https://pdbe.org/3m46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m46 RCSB], [https://www.ebi.ac.uk/pdbsum/3m46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m46 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A5ZY13_9FIRM A5ZY13_9FIRM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/3m46_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m46 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human intestine harbors a large number of microbes forming a complex microbial community that greatly affects the physiology and pathology of the host. In the human gut microbiome, the enrichment in certain protein gene families appears to be widespread. They include enzymes involved in carbohydrate metabolism such as glucoside hydrolases of dietary polysaccharides and glycoconjugates. We report the crystal structures (wild type, 2 mutants, and a mutant/substrate complex) and the enzymatic activity of a recombinant alpha-glucosidase from human gut bacterium Ruminococcus obeum. The first ever protein structures from this bacterium reveal a structural homologue to human intestinal maltase-glucoamylase with a highly conserved catalytic domain and reduced auxiliary domains. The alpha-glucosidase, a member of GH31 family, shows substrate preference for alpha(1-6) over alpha(1-4) glycosidic linkages and produces glucose from isomaltose as well as maltose. The preference can be switched by a single mutation at its active site, suggestive of widespread adaptation to utilization of a variety of polysaccharides by intestinal micro-organisms as energy resources.
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===The crystal structure of the D73A mutant of glycoside HYDROLASE (FAMILY 31) from Ruminococcus obeum ATCC 29174===
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Novel alpha-glucosidase from human gut microbiome: substrate specificities and their switch.,Tan K, Tesar C, Wilton R, Keigher L, Babnigg G, Joachimiak A FASEB J. 2010 Oct;24(10):3939-49. Epub 2010 Jun 25. PMID:20581222<ref>PMID:20581222</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3M46 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Ruminococcus_obeum Ruminococcus obeum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M46 OCA].
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<div class="pdbe-citations 3m46" style="background-color:#fffaf0;"></div>
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[[Category: Ruminococcus obeum]]
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== References ==
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[[Category: Babnigg, G.]]
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<references/>
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[[Category: Freeman, L.]]
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__TOC__
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[[Category: Joachimiak, A.]]
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</StructureSection>
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Blautia obeum ATCC 29174]]
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[[Category: Tan, K.]]
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[[Category: Large Structures]]
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[[Category: Tesar, C.]]
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[[Category: Babnigg G]]
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[[Category: Hydrolase]]
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[[Category: Freeman L]]
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[[Category: Mcsg]]
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[[Category: Joachimiak A]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Tan K]]
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[[Category: Protein structure initiative]]
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[[Category: Tesar C]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 24 09:09:11 2010''
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Current revision

The crystal structure of the D73A mutant of glycoside HYDROLASE (FAMILY 31) from Ruminococcus obeum ATCC 29174

PDB ID 3m46

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