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2x2z

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{{Seed}}
 
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[[Image:2x2z.png|left|200px]]
 
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==Crystal structure AMA1 from Toxoplasma gondii==
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The line below this paragraph, containing "STRUCTURE_2x2z", creates the "Structure Box" on the page.
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<StructureSection load='2x2z' size='340' side='right'caption='[[2x2z]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x2z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X2Z FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x2z OCA], [https://pdbe.org/2x2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x2z RCSB], [https://www.ebi.ac.uk/pdbsum/2x2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x2z ProSAT]</span></td></tr>
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{{STRUCTURE_2x2z| PDB=2x2z | SCENE= }}
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/AMA1_TOXGO AMA1_TOXGO]] Essential microneme protein that plays an important role in host cell invasion. Part of the moving junction (MJ) complex, a ringlike structure formed between the plasma membranes of the apical tip of the parasite and the target host cell. During invasion, the MJ migrates from the anterior to the posterior of the parasite, leading to internalization of the parasite into a parasitophorous vacuole (PV).<ref>PMID:11083833</ref> <ref>PMID:11087913</ref> <ref>PMID:16000372</ref> <ref>PMID:16102004</ref> <ref>PMID:16244709</ref> <ref>PMID:19247437</ref> <ref>PMID:20421941</ref> <ref>PMID:21205639</ref> <ref>PMID:22523242</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x2/2x2z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x2z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Apical membrane antigen 1 (AMA1) is an essential component of the moving junction complex used by Apicomplexan parasites to invade host cells. We report the 2.0 A resolution x-ray crystal structure of the full ectodomain (domains I, II, and III) of AMA1 from the pervasive protozoan parasite Toxoplasma gondii. The structure of T. gondii AMA1 (TgAMA1) is the most complete of any AMA1 structure to date, with more than 97.5% of the ectodomain unambiguously modeled. Comparative sequence analysis reveals discrete segments of divergence in TgAMA1 that map to areas of established functional importance in AMA1 from Plasmodium vivax (PvAMA1) and Plasmodium falciparum (PfAMA1). Inspection of the TgAMA1 structure reveals a network of apical surface loops, reorganized in both size and chemistry relative to PvAMA1/PfAMA1, that appear to serve as structural filters restricting access to a central hydrophobic groove. The terminal portion of this groove is formed by an extended loop from DII that is 14 residues shorter in TgAMA1. A pair of tryptophan residues (Trp(353) and Trp(354)) anchor the DII loop in the hydrophobic groove and frame a conserved tyrosine (Tyr(230)), forming a contiguous surface that may be critical for moving junction assembly. The minimalist DIII structure folds into a cystine knot that probably stabilizes and orients the bulk of the ectodmain without providing excess surface area to which invasion-inhibitory antibodies can be generated. The detailed structural characterization of TgAMA1 provides valuable insight into the mechanism of host cell invasion by T. gondii.
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===CRYSTAL STRUCTURE AMA1 FROM TOXOPLASMA GONDII===
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Structural characterization of apical membrane antigen 1 (AMA1) from Toxoplasma gondii.,Crawford J, Tonkin ML, Grujic O, Boulanger MJ J Biol Chem. 2010 May 14;285(20):15644-52. Epub 2010 Mar 19. PMID:20304917<ref>PMID:20304917</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20304917}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x2z" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20304917 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20304917}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2X2Z is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X2Z OCA].
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[[Category: Toxgo]]
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[[Category: Boulanger, M J]]
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==Reference==
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[[Category: Crawford, J]]
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<ref group="xtra">PMID:20304917</ref><references group="xtra"/>
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[[Category: Grujic, O]]
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[[Category: Toxoplasma gondii]]
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[[Category: Tonkin, M L]]
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[[Category: Boulanger, M J.]]
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[[Category: Crawford, J.]]
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[[Category: Grujic, O.]]
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[[Category: Tonkin, M L.]]
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[[Category: Invasion]]
[[Category: Invasion]]
[[Category: Membrane protein]]
[[Category: Membrane protein]]
[[Category: Moving junction]]
[[Category: Moving junction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 12:32:16 2010''
 

Current revision

Crystal structure AMA1 from Toxoplasma gondii

PDB ID 2x2z

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