1h21

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:27, 27 March 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1h21.jpg|left|200px]]<br /><applet load="1h21" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1h21, resolution 2.50&Aring;" />
 
-
'''A NOVEL IRON CENTRE IN THE SPLIT-SORET CYTOCHROME C FROM DESULFOVIBRIO DESULFURICANS ATCC 27774'''<br />
 
-
==Overview==
+
==A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774==
-
The facultative sulfate/nitrate-reducing bacterium Desulfovibrio, desulfuricans ATCC 27774 harbours a split-Soret cytochrome c. This, cytochrome is a homodimeric protein, having two bis-histidinyl c-type, haems per monomer. It has an unique architecture at the haem domain: each, haem has one of the coordinating histidines provided by the other monomer, and in each monomer the haems are parallel to each other, almost in van, der Waals contact. This work reports the cloning and sequencing of the, gene encoding for this cytochrome and shows, by transcriptional analysis, that it is more expressed in nitrate-grown cells than in sulfate-grown, ones. In addition, the gene-deduced amino acid sequence revealed two new, cysteine residues that could be involved in the binding of a non-haem iron, centre. Indeed, the presence of a novel type of an iron-sulfur centre, (possibly of the [2Fe-2S] type) was demonstrated by EPR spectroscopy, and, putative models for its localization and structure in the cytochrome, molecule are proposed on the basis of the so-far-known 3D crystallographic, structure of the aerobically purified split-Soret cytochrome, which lacks, this centre.
+
<StructureSection load='1h21' size='340' side='right'caption='[[1h21]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1h21]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ddc 1ddc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H21 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h21 OCA], [https://pdbe.org/1h21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h21 RCSB], [https://www.ebi.ac.uk/pdbsum/1h21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h21 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CYCX_DESDA CYCX_DESDA] Diheme cytochrome c which may be involved in sulfate reduction.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h2/1h21_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h21 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1H21 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with HEC as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1DDC. Known structural/functional Site: <scene name='pdbsite=AC1:Hec Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H21 OCA].
+
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774., Abreu IA, Lourenco AI, Xavier AV, LeGall J, Coelho AV, Matias PM, Pinto DM, Armenia Carrondo M, Teixeira M, Saraiva LM, J Biol Inorg Chem. 2003 Feb;8(3):360-70. Epub 2002 Dec 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12589573 12589573]
+
[[Category: Desulfovibrio desulfuricans]]
[[Category: Desulfovibrio desulfuricans]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Abreu, I.A.]]
+
[[Category: Abreu IA]]
-
[[Category: Carrondo, M.A.]]
+
[[Category: Carrondo MA]]
-
[[Category: Coelho, A.V.]]
+
[[Category: Coelho AV]]
-
[[Category: Legall, J.]]
+
[[Category: Legall J]]
-
[[Category: Lourenco, A.I.]]
+
[[Category: Lourenco AI]]
-
[[Category: Matias, P.M.]]
+
[[Category: Matias PM]]
-
[[Category: Pinto, D.M.]]
+
[[Category: Pinto DM]]
-
[[Category: Saraiva, L.M.]]
+
[[Category: Saraiva LM]]
-
[[Category: Teixeira, M.]]
+
[[Category: Teixeira M]]
-
[[Category: Xavier, A.V.]]
+
[[Category: Xavier AV]]
-
[[Category: HEC]]
+
-
[[Category: dimeric di-heme cytochrome]]
+
-
[[Category: electron transport]]
+
-
[[Category: novel fold]]
+
-
[[Category: novel iron-sulfur centre]]
+
-
[[Category: stacked heme arrangement]]
+
-
[[Category: sulfate respiration]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:53:09 2007''
+

Current revision

A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774

PDB ID 1h21

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h21"
Personal tools