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1h8v

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[[Image:1h8v.jpg|left|200px]]<br /><applet load="1h8v" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h8v, resolution 1.90&Aring;" />
 
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'''THE X-RAY CRYSTAL STRUCTURE OF THE TRICHODERMA REESEI FAMILY 12 ENDOGLUCANASE 3, CEL12A, AT 1.9 A RESOLUTION'''<br />
 
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==Overview==
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==The X-ray Crystal Structure of the Trichoderma reesei Family 12 Endoglucanase 3, Cel12A, at 1.9 A Resolution==
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We present the three-dimensional structure of Trichoderma reesei, endoglucanase 3 (Cel12A), a small, 218 amino acid residue (24.5 kDa), neutral pI, glycoside hydrolase family 12 cellulase that lacks a, cellulose-binding module. The structure has been determined using X-ray, crystallography and refined to 1.9 A resolution. The asymmetric unit, consists of six non-crystallographic symmetry-related molecules that were, exploited to improve initial multiple isomorphous replacement phasing, and, subsequent structure refinement. The enzyme contains one disulfide bridge, and is glycosylated at Asp164 by a single N-acetyl glucosamine residue., The protein has the expected fold for a glycoside hydrolase clan-C family, 12 enzyme. It contains two beta-sheets, of six and nine strands, packed on, top of one another, and one alpha-helix. The concave surface of the, nine-stranded beta-sheet forms a large substrate-binding groove in which, the active-site residues are located. In the active site, we find a, carboxylic acid trio, similar to that of glycoside hydrolase families 7, and 16. The strictly conserved Asp99 hydrogen bonds to the nucleophile, the invariant Glu116. The binding crevice is lined with both aromatic and, polar amino acid side-chains which may play a role in substrate binding., The structure of the fungal family 12 enzyme presented here allows a, complete structural characterization of the glycoside hydrolase-C clan.
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<StructureSection load='1h8v' size='340' side='right'caption='[[1h8v]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1h8v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8v OCA], [https://pdbe.org/1h8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8v RCSB], [https://www.ebi.ac.uk/pdbsum/1h8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O00095_HYPJE O00095_HYPJE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8v ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1H8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=CA1:The Active-Site Residues That Are Involve In Catalysis A ...'>CA1</scene>, <scene name='pdbsite=CA2:The Active-Site Residues That Are Involve In Catalysis A ...'>CA2</scene>, <scene name='pdbsite=CA3:The Active-Site Residues That Are Involve In Catalysis A ...'>CA3</scene>, <scene name='pdbsite=CA4:The Active-Site Residues That Are Involve In Catalysis A ...'>CA4</scene>, <scene name='pdbsite=CA5:The Active-Site Residues That Are Involve In Catalysis A ...'>CA5</scene> and <scene name='pdbsite=CA6:The Active-Site Residues That Are Involve In Catalysis A ...'>CA6</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8V OCA].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution., Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Stahlberg J, Mitchinson C, Jones TA, J Mol Biol. 2001 Apr 27;308(2):295-310. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11327768 11327768]
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[[Category: Large Structures]]
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[[Category: Cellulase]]
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[[Category: Trichoderma reesei]]
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[[Category: Hypocrea jecorina]]
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[[Category: Bott R]]
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[[Category: Single protein]]
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[[Category: Cameron AD]]
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[[Category: Bott, R.]]
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[[Category: Jones TA]]
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[[Category: Cameron, A.D.]]
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[[Category: Mitchinson C]]
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[[Category: Jones, T.A.]]
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[[Category: Ropp TH]]
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[[Category: Mitchinson, C.]]
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[[Category: Sandgren M]]
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[[Category: Ropp, T.H.]]
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[[Category: Shaw A]]
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[[Category: Sandgren, M.]]
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[[Category: Stahlberg J]]
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[[Category: Shaw, A.]]
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[[Category: Wu S]]
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[[Category: Stahlberg, J.]]
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[[Category: Wu, S.]]
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[[Category: NAG]]
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[[Category: cellulase]]
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[[Category: cellulose degradation]]
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[[Category: endoglucanase]]
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[[Category: gh family 12]]
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[[Category: glycosyl hydrolase]]
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[[Category: hydrolase]]
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[[Category: trichoderma reesei cel12a]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:08:45 2007''
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Current revision

The X-ray Crystal Structure of the Trichoderma reesei Family 12 Endoglucanase 3, Cel12A, at 1.9 A Resolution

PDB ID 1h8v

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