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1h9h
From Proteopedia
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| - | [[Image:1h9h.jpg|left|200px]]<br /><applet load="1h9h" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1h9h, resolution 1.5Å" /> | ||
| - | '''COMPLEX OF EETI-II WITH PORCINE TRYPSIN'''<br /> | ||
| - | == | + | ==COMPLEX OF EETI-II WITH PORCINE TRYPSIN== |
| - | + | <StructureSection load='1h9h' size='340' side='right'caption='[[1h9h]], [[Resolution|resolution]] 1.50Å' scene=''> | |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1h9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H9H FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9h OCA], [https://pdbe.org/1h9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h9h RCSB], [https://www.ebi.ac.uk/pdbsum/1h9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9h_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9h ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold. | ||
| + | |||
| + | Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.,Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759<ref>PMID:16131759</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 1h9h" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Trypsin 3D structures|Trypsin 3D structures]] | ||
| + | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Ecballium elaterium]] | [[Category: Ecballium elaterium]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
| - | + | [[Category: Kolmar H]] | |
| - | [[Category: Kolmar | + | [[Category: Kraetzner R]] |
| - | [[Category: Kraetzner | + | [[Category: Uson I]] |
| - | [[Category: Uson | + | [[Category: Wentzel A]] |
| - | [[Category: Wentzel | + | |
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Current revision
COMPLEX OF EETI-II WITH PORCINE TRYPSIN
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