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2fay

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{{Theoretical_model}}
{{Theoretical_model}}
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{{Seed}}
 
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[[Image:2fay.png|left|200px]]
 
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==THEORETICAL MODEL OF THE PICHIA PASTORIS SPHINGOLIPID C9- METHYLTRANSFERASE==
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The line below this paragraph, containing "STRUCTURE_2fay", creates the "Structure Box" on the page.
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<StructureSection load='2fay' size='340' side='right'caption='[[2fay]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FAY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fay FirstGlance], [https://www.ebi.ac.uk/pdbsum/2fay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fay ProSAT]</span></td></tr>
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</table>
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{{STRUCTURE_2fay| PDB=2fay | SCENE= }}
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Fungal glucosylceramides play an important role in plant-pathogen interactions enabling plants to recognize the fungal attack and initiate specific defense responses. A prime structural feature distinguishing fungal glucosylceramides from those of plants and animals is a methyl group at the C9-position of the sphingoid base, the biosynthesis of which has never been investigated. Using information on the presence or absence of C9-methylated glucosylceramides in different fungal species, we developed a bioinformatics strategy to identify the gene responsible for the biosynthesis of this C9-methyl group. This phylogenetic profiling allowed the selection of a single candidate out of 24-71 methyltransferase sequences present in each of the fungal species with C9-methylated glucosylceramides. A Pichia pastoris knock-out strain lacking the candidate sphingolipid C9-methyltransferase was generated, and indeed, this strain contained only non-methylated glucosylceramides. In a complementary approach, a Saccharomyces cerevisiae strain was engineered to produce glucosylceramides suitable as a substrate for C9-methylation. C9-methylated sphingolipids were detected in this strain expressing the candidate from P. pastoris, demonstrating its function as a sphingolipid C9-methyltransferase. The enzyme belongs to the superfamily of S-adenosylmethionine-(SAM)-dependent methyltransferases and shows highest sequence similarity to plant and bacterial cyclopropane fatty acid synthases. An in vitro assay showed that sphingolipid C9-methylation is membrane-bound and requires SAM and Delta4,8-desaturated ceramide as substrates.
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===THEORETICAL MODEL OF THE PICHIA PASTORIS SPHINGOLIPID C9- METHYLTRANSFERASE===
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Identification of fungal sphingolipid C9-methyltransferases by phylogenetic profiling.,Ternes P, Sperling P, Albrecht S, Franke S, Cregg JM, Warnecke D, Heinz E J Biol Chem. 2006 Mar 3;281(9):5582-92. Epub 2005 Dec 8. PMID:16339149<ref>PMID:16339149</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16339149}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2fay" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16339149 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16339149}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Theoretical Model]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FAY OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:16339149</ref><references group="xtra"/>
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[[Category: Grinstead, J S]]
[[Category: Grinstead, J S]]
[[Category: Ternes, P]]
[[Category: Ternes, P]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:45:11 2010''
 

Current revision

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

THEORETICAL MODEL OF THE PICHIA PASTORIS SPHINGOLIPID C9- METHYLTRANSFERASE

PDB ID 2fay

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