1hkq

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[[Image:1hkq.gif|left|200px]]<br /><applet load="1hkq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hkq, resolution 2.75&Aring;" />
 
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'''PPS10 PLASMID DNA REPLICATION INITIATOR PROTEIN REPA. REPLICATION INACTIVE, DIMERIC N-TERMINAL DOMAIN.'''<br />
 
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==Overview==
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==PPS10 plasmid DNA replication initiator protein RepA. Replication inactive, dimeric N-terminal domain.==
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Plasmids are natural vectors for gene transfer. In Gram-negative bacteria, plasmid DNA replication is triggered when monomers of an initiator protein, (Rep) bind to direct repeats at the origin sequence. Rep dimers, which are, inactive as initiators, bind to an inverse repeat operator, repressing, transcription of the rep gene. Rep proteins are composed of N-terminal, dimerization and C-terminal DNA-binding domains. Activation of Rep is, coupled to dimer dissociation, converting the dimerization domain into a, second origin-binding module. Although the structure of the monomeric F, plasmid initiator (mRepE) has been determined, the molecular nature of Rep, activation remains unknown. Here we report the crystal structure of the, dimeric N-terminal domain of the pPS10 plasmid initiator (dRepA). dRepA, has a winged-helix fold, as does its homologous domain in mRepE. However, dimerization transforms an interdomain loop and beta-strand (monomeric, RepE) into an alpha-helix (dimeric RepA). dRepA resemble the C terminus of, eukaryotic and archaeal Cdc6, giving clues to the phylogeny of DNA, replication initiators.
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<StructureSection load='1hkq' size='340' side='right'caption='[[1hkq]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hkq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_savastanoi Pseudomonas savastanoi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKQ FirstGlance]. <br>
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1HKQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_savastanoi Pseudomonas savastanoi] with HG, PO4 and BEZ as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Hg Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkq OCA], [https://pdbe.org/1hkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hkq RCSB], [https://www.ebi.ac.uk/pdbsum/1hkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkq ProSAT]</span></td></tr>
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A conformational switch between transcriptional repression and replication initiation in the RepA dimerization domain., Giraldo R, Fernandez-Tornero C, Evans PR, Diaz-Orejas R, Romero A, Nat Struct Biol. 2003 Jul;10(7):565-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12766757 12766757]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q52546_PSESX Q52546_PSESX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hkq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas savastanoi]]
[[Category: Pseudomonas savastanoi]]
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[[Category: Single protein]]
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[[Category: Diaz-Orejas R]]
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[[Category: Diaz-Orejas, R.]]
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[[Category: Evans PR]]
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[[Category: Evans, P.R.]]
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[[Category: Fernandez-Tornero C]]
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[[Category: Fernandez-Tornero, C.]]
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[[Category: Giraldo R]]
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[[Category: Giraldo, R.]]
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[[Category: Romero A]]
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[[Category: Romero, A.]]
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[[Category: BEZ]]
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[[Category: HG]]
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[[Category: PO4]]
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[[Category: dna binding protein]]
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[[Category: pps10 plasmid]]
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[[Category: replication initiator dimer.]]
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[[Category: winged-helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:30:39 2007''
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Current revision

PPS10 plasmid DNA replication initiator protein RepA. Replication inactive, dimeric N-terminal domain.

PDB ID 1hkq

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