1l4o

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{{Theoretical_model}}
{{Theoretical_model}}
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{{Seed}}
 
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[[Image:1l4o.png|left|200px]]
 
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==HEXAMER OF DNA-PACKAGING RNA (PRNA) OF BACTERIOPHAGE PHI29 DNA-TRANSLOCATING MOTOR==
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The line below this paragraph, containing "STRUCTURE_1l4o", creates the "Structure Box" on the page.
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<StructureSection load='1l4o' size='340' side='right'caption='[[1l4o]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L4O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l4o FirstGlance], [https://www.ebi.ac.uk/pdbsum/1l4o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l4o ProSAT]</span></td></tr>
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</table>
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{{STRUCTURE_1l4o| PDB=1l4o | SCENE= }}
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A striking common feature in the maturation of all linear double-stranded DNA viruses is that their lengthy genome is translocated with remarkable velocity into the limited space within a preformed protein shell and packaged into near crystalline density. A DNA-translocating motor, powered by ATP hydrolysis, accomplishes this task, which would otherwise be energetically unfavorable. DNA-packaging RNA, pRNA, forms a hexameric complex to serve as a vital component of the DNA translocating motor of bacterial virus Phi29. The sequential action of six pRNA ensures continual function in the DNA translocation process. The Phi29 motor has been assembled with purified components synthesized by chemical or biotechnological approaches and is able to pump the viral DNA into the protein shell in vitro. pRNA dimers are the building blocks of the hexamer. The computer models of the three-dimensional structure of the motor was constructed based on experimental data derived from photoaffinity cross-linking by psoralen, phenphi (cis-Rh(1,10-phenanthroline)(9,10-phenan-threnequinone diimine)Cl(2)(+)), and azidophenacyl; chemical modification and chemical modification interference with dimethyl sulfate, 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluene sulfonate, and kethoxal; complementary modification; and nuclease probing by single- and double-stranded specific RNases. The shapes of these computer models are very similar to the published pRNA images of cryo-atomic force microscopy. pRNA hexamer docking with the connector crystal structure reveals a very impressive match with the available biochemical, genetic, and physical data.
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===HEXAMER OF DNA-PACKAGING RNA (PRNA) OF BACTERIOPHAGE PHI29 DNA-TRANSLOCATING MOTOR===
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Computer modeling of three-dimensional structure of DNA-packaging RNA (pRNA) monomer, dimer, and hexamer of Phi29 DNA packaging motor.,Hoeprich S, Guo P J Biol Chem. 2002 Jun 7;277(23):20794-803. Epub 2002 Mar 8. PMID:11886855<ref>PMID:11886855</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11886855}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1l4o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11886855 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11886855}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Theoretical Model]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L4O OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:11886855</ref><references group="xtra"/>
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[[Category: Guo, P]]
[[Category: Guo, P]]
[[Category: Hoeprich, S]]
[[Category: Hoeprich, S]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 08:36:28 2010''
 

Current revision

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

HEXAMER OF DNA-PACKAGING RNA (PRNA) OF BACTERIOPHAGE PHI29 DNA-TRANSLOCATING MOTOR

PDB ID 1l4o

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