2x53

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{{Seed}}
 
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[[Image:2x53.png|left|200px]]
 
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==Structure of the phage p2 baseplate in its activated conformation with Sr==
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The line below this paragraph, containing "STRUCTURE_2x53", creates the "Structure Box" on the page.
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<StructureSection load='2x53' size='340' side='right'caption='[[2x53]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x53]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_virus_P2 Lactococcus virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X53 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
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{{STRUCTURE_2x53| PDB=2x53 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x53 OCA], [https://pdbe.org/2x53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x53 RCSB], [https://www.ebi.ac.uk/pdbsum/2x53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x53 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D3KFX5_BPLP2 D3KFX5_BPLP2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x5/2x53_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x53 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Siphoviridae is the most abundant viral family on earth which infects bacteria as well as archaea. All known siphophages infecting gram+ Lactococcus lactis possess a baseplate at the tip of their tail involved in host recognition and attachment. Here, we report analysis of the p2 phage baseplate structure by X-ray crystallography and electron microscopy and propose a mechanism for the baseplate activation during attachment to the host cell. This approximately 1 MDa, Escherichia coli-expressed baseplate is composed of three protein species, including six trimers of the receptor-binding protein (RBP). RBPs host-recognition domains point upwards, towards the capsid, in agreement with the electron-microscopy map of the free virion. In the presence of Ca(2+), a cation mandatory for infection, the RBPs rotated 200 degrees downwards, presenting their binding sites to the host, and a channel opens at the bottom of the baseplate for DNA passage. These conformational changes reveal a novel siphophage activation and host-recognition mechanism leading ultimately to DNA ejection.
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===STRUCTURE OF THE PHAGE P2 BASEPLATE IN ITS ACTIVATED CONFORMATION WITH SR===
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Structure of lactococcal phage p2 baseplate and its mechanism of activation.,Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau C Proc Natl Acad Sci U S A. 2010 Mar 29. PMID:20351260<ref>PMID:20351260</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20351260}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x53" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20351260 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20351260}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Lactococcus virus P2]]
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2X53 is a 27 chains structure with sequences from [http://en.wikipedia.org/wiki/Lactococcus_phage_p2 Lactococcus phage p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X53 OCA].
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[[Category: Large Structures]]
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[[Category: Bebeacua C]]
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==Reference==
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[[Category: Bron P]]
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<ref group="xtra">PMID:20351260</ref><references group="xtra"/>
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[[Category: Cambillau C]]
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[[Category: Lactococcus phage p2]]
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[[Category: Campanacci V]]
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[[Category: Bebeacua, C.]]
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[[Category: Lichiere J]]
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[[Category: Bron, P.]]
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[[Category: Moineau S]]
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[[Category: Cambillau, C.]]
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[[Category: Ortiz-Lombardia M]]
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[[Category: Campanacci, V.]]
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[[Category: Sciara G]]
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[[Category: Lichiere, J.]]
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[[Category: Tremblay D]]
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[[Category: Moineau, S.]]
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[[Category: Van Heel M]]
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[[Category: Ortiz-Lombardia, M.]]
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[[Category: Sciara, G.]]
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[[Category: Tremblay, D.]]
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[[Category: Vanheel, M.]]
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[[Category: Baseplate]]
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[[Category: Viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 08:57:51 2010''
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Current revision

Structure of the phage p2 baseplate in its activated conformation with Sr

PDB ID 2x53

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OCA

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