2wq5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:35, 6 November 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2wq5.png|left|200px]]
 
-
<!--
+
==Non-antibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.==
-
The line below this paragraph, containing "STRUCTURE_2wq5", creates the "Structure Box" on the page.
+
<StructureSection load='2wq5' size='340' side='right'caption='[[2wq5]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2wq5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_naja Naja naja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WQ5 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MIY:(4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-CARBOXAMIDE'>MIY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_2wq5| PDB=2wq5 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wq5 OCA], [https://pdbe.org/2wq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wq5 RCSB], [https://www.ebi.ac.uk/pdbsum/2wq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wq5 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PA2A2_NAJNA PA2A2_NAJNA] PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/2wq5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wq5 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Secretory phospholipase A(2) is involved in inflammatory processes and was previously shown to be inhibited by lipophilic tetracyclines such as minocycline (minoTc) and doxycycline. Lipophilic tetracyclines might be a new lead compound for the design of specific inhibitors of secretory phospholipase A(2), which play a crucial role in inflammatory processes. Our X-ray crystal structure analysis at 1.65 A resolution of the minoTc complex of phospholipase A(2) (PLA(2)) of the Indian cobra (Naja naja naja) is the first example of nonantibiotic tetracycline interactions with a protein. MinoTc interferes with the conformation of the active-site Ca(2+)-binding loop, preventing Ca(2)(+) binding, and shields the active site from substrate entrance, resulting in inhibition of the enzyme. MinoTc binding to PLA(2) is dominated by hydrophobic interactions quite different from antibiotic recognition of tetracyclines by proteins or the ribosome. The affinity of minoTc for PLA(2) was determined by surface plasmon resonance, resulting in a dissociation constant K(d)=1.8 x 10(-)(4) M.
-
===NON-ANTIBIOTIC PROPERTIES OF TETRACYCLINES: STRUCTURAL BASIS FOR INHIBITION OF SECRETORY PHOSPHOLIPASE A2.===
+
Nonantibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.,Dalm D, Palm GJ, Aleksandrov A, Simonson T, Hinrichs W J Mol Biol. 2010 Apr 23;398(1):83-96. Epub 2010 Mar 6. PMID:20211188<ref>PMID:20211188</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2wq5" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_20211188}}, adds the Publication Abstract to the page
+
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 20211188 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_20211188}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2WQ5 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_naja Naja naja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WQ5 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:20211188</ref><references group="xtra"/>
+
[[Category: Naja naja]]
[[Category: Naja naja]]
-
[[Category: Dalm, D.]]
+
[[Category: Dalm D]]
-
[[Category: Hinrichs, W.]]
+
[[Category: Hinrichs W]]
-
[[Category: Palm, G J.]]
+
[[Category: Palm GJ]]
-
[[Category: Calcium binding loop]]
+
-
[[Category: Carboxylic ester hydrolase]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Lipid degradation]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Minocycline]]
+
-
[[Category: Phospholipase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 09:09:25 2010''
+

Current revision

Non-antibiotic properties of tetracyclines: structural basis for inhibition of secretory phospholipase A2.

PDB ID 2wq5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wq5"
Personal tools