2x8x

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{{Seed}}
 
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[[Image:2x8x.png|left|200px]]
 
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==Structure of the N-terminal domain of Omp85 from the Thermophilic Cyanobacterium Thermosynechococcus elongatus==
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The line below this paragraph, containing "STRUCTURE_2x8x", creates the "Structure Box" on the page.
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<StructureSection load='2x8x' size='340' side='right'caption='[[2x8x]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x8x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus Thermosynechococcus vestitus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8x OCA], [https://pdbe.org/2x8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8x RCSB], [https://www.ebi.ac.uk/pdbsum/2x8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8x ProSAT]</span></td></tr>
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{{STRUCTURE_2x8x| PDB=2x8x | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8DI03_THEVB Q8DI03_THEVB]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Omp85 proteins are essential proteins located in the bacterial outer membrane. They are involved in outer membrane biogenesis and assist outer membrane protein insertion and folding by an unknown mechanism. Homologous proteins exist in eukaryotes, where they mediate outer membrane assembly in organelles of endosymbiotic origin, the mitochondria and chloroplasts. We set out to explore the homologous relationship between cyanobacteria and chloroplasts, studying the Omp85 protein from the thermophilic cyanobacterium Thermosynechococcus elongatus. Using state-of-the art sequence analysis and clustering methods, we show how this protein is more closely related to its chloroplast homologue Toc75 than to proteobacterial Omp85, a finding supported by single channel conductance measurements. We have solved the structure of the periplasmic part of the protein to 1.97 A resolution, and we demonstrate that in contrast to Omp85 from Escherichia coli the protein has only three, not five, polypeptide transport-associated (POTRA) domains, which recognize substrates and generally interact with other proteins in bigger complexes. We model how these POTRA domains are attached to the outer membrane, based on the relationship of Omp85 to two-partner secretion system proteins, which we show and analyze. Finally, we discuss how Omp85 proteins with different numbers of POTRA domains evolved, and evolve to this day, to accomplish an increasing number of interactions with substrates and helper proteins.
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===STRUCTURE OF THE N-TERMINAL DOMAIN OF OMP85 FROM THE THERMOPHILIC CYANOBACTERIUM THERMOSYNECHOCOCCUS ELONGATUS===
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Omp85 from the thermophilic cyanobacterium Thermosynechococcus elongatus differs from proteobacterial Omp85 in structure and domain composition.,Arnold T, Zeth K, Linke D J Biol Chem. 2010 Jun 4;285(23):18003-15. Epub 2010 Mar 29. PMID:20351097<ref>PMID:20351097</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20351097}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x8x" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20351097 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20351097}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2X8X is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8X OCA].
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[[Category: Thermosynechococcus vestitus]]
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[[Category: Arnold T]]
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==Reference==
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[[Category: Linke D]]
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<ref group="xtra">PMID:20351097</ref><references group="xtra"/>
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[[Category: Zeth K]]
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[[Category: Thermosynechococcus elongatus]]
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[[Category: Arnold, T.]]
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[[Category: Linke, D.]]
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[[Category: Zeth, K.]]
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[[Category: Chaperone]]
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[[Category: Protein targeting]]
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[[Category: Toc75]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 09:09:34 2010''
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Current revision

Structure of the N-terminal domain of Omp85 from the Thermophilic Cyanobacterium Thermosynechococcus elongatus

PDB ID 2x8x

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