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1ocs

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[[Image:1ocs.jpg|left|200px]]<br /><applet load="1ocs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ocs, resolution 2.03&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE YEAST PX-DOAMIN PROTEIN GRD19P (SORTING NEXIN3) COMPLEXED TO PHOSPHATIDYLINOSYTOL-3-PHOSPHATE.'''<br />
 
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==Overview==
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==Crystal structure of the yeast PX-doamin protein Grd19p (sorting nexin3) complexed to phosphatidylinosytol-3-phosphate.==
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Phox homology (PX) domains have been recently identified in a number of, different proteins and are involved in various cellular functions such as, vacuolar targeting and membrane protein trafficking. It was shown that, these modules of about 130 amino acids specifically binding to, phosphoinositides and that this interaction is crucial for their cellular, function. The yeast genome contains 17 PX domain proteins. One of these, Grd19p, is involved in the localization of the late Golgi membrane, proteins DPAP A and Kex2p. Grd19p consists of the PX domain with 30 extra, residues at the N-terminal and is homologous to the functionally, characterized human sorting nexin protein SNX3. We determined the 2.0 A, crystal structure of Grd19p in the free form and in complex with, d-myo-phosphatidylinositol 3-phosphate (diC4PtdIns(3)P), representing the, first case of both free and ligand-bound conformations of the same PX, module. The ligand occupies a well defined positively charged binding, pocket at the interface between the beta-sheet and alpha-helical parts of, the molecule. The structure of the free and bound protein are globally, similar but show some significant differences in a region containing a, polyproline peptide and a putative membrane attachment site.
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<StructureSection load='1ocs' size='340' side='right'caption='[[1ocs]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ocs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OCS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ocs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocs OCA], [https://pdbe.org/1ocs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ocs RCSB], [https://www.ebi.ac.uk/pdbsum/1ocs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ocs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNX3_YEAST SNX3_YEAST] Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).<ref>PMID:9456318</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/1ocs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ocs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OCS OCA].
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*[[Sorting nexin 3D structures|Sorting nexin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate., Zhou CZ, de La Sierra-Gallay IL, Quevillon-Cheruel S, Collinet B, Minard P, Blondeau K, Henckes G, Aufrere R, Leulliot N, Graille M, Sorel I, Savarin P, de la Torre F, Poupon A, Janin J, van Tilbeurgh H, J Biol Chem. 2003 Dec 12;278(50):50371-6. Epub 2003 Sep 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14514667 14514667]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Aufrere R]]
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[[Category: Aufrere, R.]]
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[[Category: Blondeau K]]
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[[Category: Blondeau, K.]]
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[[Category: Cheruel S]]
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[[Category: Cheruel, S.]]
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[[Category: Collinet B]]
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[[Category: Collinet, B.]]
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[[Category: De La Torre F]]
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[[Category: Graille, M.]]
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[[Category: Graille M]]
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[[Category: Henkes, G.]]
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[[Category: Henkes G]]
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[[Category: Janin, J.]]
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[[Category: Janin J]]
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[[Category: Leulliot, N.]]
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[[Category: Leulliot N]]
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[[Category: Minard, P.]]
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[[Category: Li De La Sierra-Gallay I]]
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[[Category: Poupon, A.]]
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[[Category: Minard P]]
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[[Category: Savarin, P.]]
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[[Category: Poupon A]]
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[[Category: Sierra-Gallay, I.Li.De.La.]]
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[[Category: Savarin P]]
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[[Category: Sorel, I.]]
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[[Category: Sorel I]]
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[[Category: Tilbeurgh, H.Van.]]
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[[Category: Van Tilbeurgh H]]
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[[Category: Torre, F.De.La.]]
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[[Category: Zhou CZ]]
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[[Category: Zhou, C.Z.]]
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[[Category: GOL]]
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[[Category: px-domain]]
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[[Category: sorting nexin]]
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[[Category: yeast protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:25:17 2007''
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Current revision

Crystal structure of the yeast PX-doamin protein Grd19p (sorting nexin3) complexed to phosphatidylinosytol-3-phosphate.

PDB ID 1ocs

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