3k1b

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{{Seed}}
 
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[[Image:3k1b.jpg|left|200px]]
 
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==Structure of OmpF porin==
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The line below this paragraph, containing "STRUCTURE_3k1b", creates the "Structure Box" on the page.
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<StructureSection load='3k1b' size='340' side='right'caption='[[3k1b]], [[Resolution|resolution]] 4.39&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3k1b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K1B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.39&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k1b OCA], [https://pdbe.org/3k1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k1b RCSB], [https://www.ebi.ac.uk/pdbsum/3k1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k1b ProSAT]</span></td></tr>
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{{STRUCTURE_3k1b| PDB=3k1b | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OMPF_ECOLI OMPF_ECOLI] Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2.<ref>PMID:19721064</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k1/3k1b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k1b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The endogenous Escherichia coli porin OmpF was crystallized as an accidental by-product of our efforts to express, purify and crystallize the Escherichia coli integral membrane protein KdpD in the presence of foscholine-12. Foscholine-12 is widely used in membrane protein studies, but no crystal structure of a protein that was both purified and crystallized with this detergent has been reported in PDB. Crystallization screening for KdpD yielded two different crystals of contaminating protein OmpF. Here we report two OmpF structures, the first membrane protein crystal structures for which extraction, purification and crystallization were done exclusively with foscholine-12. The first structure was refined in space group P21 with cell parameters a = 136.7 A, b = 210.5 A, c = 137 A and beta = 100.5 degrees , and the resolution of 3.8 A. The second structure was solved at the resolution of 4.4 A and was refined in the P321 space group, with unit-cell parameters a = 215.5 A, b = 215.5 A, c = 137.5 A and gamma = 120 degrees . Both crystal forms show novel crystal packing, in which the building block is a tetrahedral arrangement of four trimers. Additionally we discuss the use of foscholine-12 for membrane protein crystallization and structure determination, as well as the problem of the OmpF contamination for membrane proteins overexpressed in E. coli.
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===Structure of OmpF porin===
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Structures of the OmpF porin crystallized in the presence of foscholine-12.,Kefala G, Ahn C, Krupa M, Esquivies L, Maslennikov I, Kwiatkowski W, Choe S Protein Sci. 2010 Mar 1. PMID:20196071<ref>PMID:20196071</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3k1b" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20196071}}, adds the Publication Abstract to the page
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*[[Porin 3D structures|Porin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20196071 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20196071}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3K1B is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K1B OCA].
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[[Category: Ahn C]]
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[[Category: Choe S]]
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==Reference==
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[[Category: Kefala G]]
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<ref group="xtra">PMID:20196071</ref><references group="xtra"/>
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[[Category: Krupa M]]
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[[Category: Escherichia coli]]
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[[Category: Kwiatkowski W]]
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[[Category: Ahn, C.]]
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[[Category: Maslennikov I]]
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[[Category: CSMP, Center for Structures of Membrane Proteins.]]
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[[Category: Choe, S.]]
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[[Category: Kefala, G.]]
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[[Category: Krupa, M.]]
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[[Category: Kwiatkowski, W.]]
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[[Category: Maslennikov, I.]]
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[[Category: Cell membrane]]
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[[Category: Cell outer membrane]]
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[[Category: Center for structures of membrane protein]]
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[[Category: Csmp]]
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[[Category: Foscholine-12]]
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[[Category: Ion transport]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: Ompf porin]]
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[[Category: Phage recognition]]
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[[Category: Porin]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 09:40:47 2010''
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Current revision

Structure of OmpF porin

PDB ID 3k1b

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