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1ogq

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[[Image:1ogq.jpg|left|200px]]<br /><applet load="1ogq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ogq, resolution 1.7&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF PGIP (POLYGALACTURONASE INHIBITING PROTEIN), A LEUCINE RICH REPEAT PROTEIN INVOLVED IN PLANT DEFENSE'''<br />
 
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==Overview==
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==The crystal structure of PGIP (polygalacturonase inhibiting protein), a leucine rich repeat protein involved in plant defense==
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Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall proteins, that protect plants from fungal invasion. They interact with, endopolygalacturonases secreted by phytopathogenic fungi, inhibit their, enzymatic activity, and favor the accumulation of oligogalacturonides, which activate plant defense responses. PGIPs are members of the, leucine-rich repeat (LRR) protein family that in plants play crucial roles, in development, defense against pathogens, and recognition of beneficial, microbes. Here we report the crystal structure at 1.7-A resolution of a, PGIP from Phaseolus vulgaris. The structure is characterized by the, presence of two beta-sheets instead of the single one originally predicted, by modeling studies. The structure also reveals a negatively charged, surface on the LRR concave face, likely involved in binding, polygalacturonases. The structural information on PGIP provides a basis, for designing more efficient inhibitors for plant protection.
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<StructureSection load='1ogq' size='340' side='right'caption='[[1ogq]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ogq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OGQ FirstGlance]. <br>
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1OGQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris] with NAG and ACT as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Act Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ogq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ogq OCA], [https://pdbe.org/1ogq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ogq RCSB], [https://www.ebi.ac.uk/pdbsum/1ogq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ogq ProSAT]</span></td></tr>
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The crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense., Di Matteo A, Federici L, Mattei B, Salvi G, Johnson KA, Savino C, De Lorenzo G, Tsernoglou D, Cervone F, Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):10124-8. Epub 2003 Aug 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12904578 12904578]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGIP2_PHAVU PGIP2_PHAVU] Inhibitor of fungal polygalacturonase. It is an important factor for plant resistance to phytopathogenic fungi. Inhibits all polygalacturonases (PG) tested, with the exception of PG from F.oxysporum which was only inhibited at 60%.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/og/1ogq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ogq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Phaseolus vulgaris]]
[[Category: Phaseolus vulgaris]]
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[[Category: Single protein]]
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[[Category: Cervone F]]
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[[Category: Cervone, F.]]
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[[Category: De Lorenzo G]]
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[[Category: Federici, L.]]
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[[Category: Di Matteo A]]
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[[Category: Johnson, K.A.]]
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[[Category: Federici L]]
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[[Category: Lorenzo, G.De.]]
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[[Category: Johnson KA]]
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[[Category: Mattei, B.]]
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[[Category: Mattei B]]
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[[Category: Matteo, A.Di.]]
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[[Category: Salvi G]]
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[[Category: Salvi, G.]]
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[[Category: Savino C]]
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[[Category: Savino, C.]]
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[[Category: Tsernoglou D]]
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[[Category: Tsernoglou, D.]]
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[[Category: ACT]]
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[[Category: NAG]]
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[[Category: inhibitor]]
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[[Category: polygalacturonase inhibiting protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:42:33 2007''
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Current revision

The crystal structure of PGIP (polygalacturonase inhibiting protein), a leucine rich repeat protein involved in plant defense

PDB ID 1ogq

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