3ih2

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{{Seed}}
 
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[[Image:3ih2.png|left|200px]]
 
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==TM1030 crystallized at 323K==
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The line below this paragraph, containing "STRUCTURE_3ih2", creates the "Structure Box" on the page.
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<StructureSection load='3ih2' size='340' side='right'caption='[[3ih2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ih2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IH2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3ih2| PDB=3ih2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ih2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ih2 OCA], [https://pdbe.org/3ih2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ih2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ih2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ih2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9X0C0_THEMA Q9X0C0_THEMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ih/3ih2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ih2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transcriptional regulator protein TM1030 from the hyperthermophile Thermotoga maritima, as well as its complex with DNA, was crystallized at a wide range of temperatures. Crystallization plates were incubated at 4, 20, 37 and 50 degrees C over 3 weeks. The best crystals of TM1030 in complex with DNA were obtained at 4, 20 and 37 degrees C, while TM1030 alone crystallized almost equally well in all temperatures. The crystals grown at different temperatures were used for X-ray diffraction experiments and their structures were compared. Surprisingly, the models of TM1030 obtained from crystals grown at different temperatures are similar in quality. While there are some examples of structures of proteins grown at elevated temperatures in the PDB, these temperatures appear to be underrepresented. Our studies show that crystals of some proteins may be grown and are stable at broad range of temperatures. We suggest that crystallization experiments at elevated temperatures could be used as a standard part of the crystallization protocol.
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===TM1030 crystallized at 323K===
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'Hot' macromolecular crystals.,Koclega KD, Chruszcz M, Zimmerman MD, Bujacz G, Minor W Cryst Growth Des. 2009 Dec 18;10(2):580. PMID:20161694<ref>PMID:20161694</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ih2" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20161694}}, adds the Publication Abstract to the page
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*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20161694 is the PubMed ID number.
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20161694}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3IH2 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IH2 OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:20161694</ref><references group="xtra"/>
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Bujacz, G.]]
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[[Category: Bujacz G]]
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[[Category: Chruszcz, M.]]
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[[Category: Chruszcz M]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Koclega, K D.]]
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[[Category: Koclega KD]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Minor W]]
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[[Category: Minor, W.]]
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[[Category: Dna-binding]]
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[[Category: Mcsg]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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[[Category: Temperature]]
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[[Category: Tm1030]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcription regulator]]
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[[Category: Transcriptional regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 21 09:09:09 2010''
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Current revision

TM1030 crystallized at 323K

PDB ID 3ih2

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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