3jqz

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{{Seed}}
 
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[[Image:3jqz.jpg|left|200px]]
 
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==Crystal Structure of Human serum albumin complexed with Lidocaine==
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The line below this paragraph, containing "STRUCTURE_3jqz", creates the "Structure Box" on the page.
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<StructureSection load='3jqz' size='340' side='right'caption='[[3jqz]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3jqz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JQZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LQZ:2-(DIETHYLAMINO)-N-(2,6-DIMETHYLPHENYL)ETHANAMIDE'>LQZ</scene></td></tr>
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{{STRUCTURE_3jqz| PDB=3jqz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqz OCA], [https://pdbe.org/3jqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jqz RCSB], [https://www.ebi.ac.uk/pdbsum/3jqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jqz ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[https://omim.org/entry/103600 103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/3jqz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jqz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human serum albumin (HSA), the major protein component in blood plasma and in extravascular spaces, is known to participate in the binding and transport of a variety of endogenous and exogenous organic compounds with anionic or electronegative features. We here report on the 3.3A resolution crystal structure of HSA complexed with the cationic, and widely used, anesthetic lidocaine. We find that lidocaine and HSA co-crystallise as a dimer in the unusual space group I4(1). The dimer consists of one HSA molecule without ligand and one HSA molecule with a single, bound lidocaine. HSA is a heart-shaped protein composed of three homologous helical domains (I-III), which can be subdivided into two subdomains (A and B), and lidocaine binds to a unique site formed by residues from subdomain IB facing the central, interdomain crevice. In the crystal, binding seems to introduce only local conformational changes in the protein. According to intrinsic fluorescence experiments with aqueous HSA binding results in widespread conformational changes involving Trp214 in subdomain IIA. Results obtained with equilibrium dialysis and isothermal titration calorimetry show that lidocaine binding is of a low affinity and occurs at one discrete binding site in accordance with the X-ray data. Another crystal form of ligand-free HSA obtained in the presence of ammonium sulphate was determined at 2.3A resolution revealing a sulphate ion accepting cavity at the surface of subdomain IIIA. The present results contribute to a further characterisation of the exceptional binding properties of HSA.
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===Crystal Structure of Human serum albumin complexed with Lidocaine===
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Crystallographic analysis reveals a unique lidocaine binding site on human serum albumin.,Hein KL, Kragh-Hansen U, Morth JP, Jeppesen MD, Otzen D, Moller JV, Nissen P J Struct Biol. 2010 Sep;171(3):353-60. Epub 2010 Mar 27. PMID:20347991<ref>PMID:20347991</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3jqz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20347991}}, adds the Publication Abstract to the page
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*[[Albumin 3D structures|Albumin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20347991 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20347991}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3JQZ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQZ OCA].
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==Reference==
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<ref group="xtra">PMID:20347991</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Hein, K L.]]
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[[Category: Large Structures]]
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[[Category: Kragh-Hansen, U.]]
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[[Category: Hein KL]]
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[[Category: Morth, J P.]]
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[[Category: Kragh-Hansen U]]
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[[Category: Nissen, P.]]
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[[Category: Morth JP]]
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[[Category: Albumin]]
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[[Category: Nissen P]]
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[[Category: Alternative splicing]]
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[[Category: Carrier protein]]
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[[Category: Cleavage on pair of basic residue]]
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[[Category: Copper]]
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[[Category: Disease mutation]]
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[[Category: Disulfide bond]]
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[[Category: Drug-binding]]
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[[Category: Glycation]]
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[[Category: Glycoprotein]]
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[[Category: Lidocaine]]
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[[Category: Lipid-binding]]
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[[Category: Metal-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Secreted]]
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[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 21 10:04:30 2010''
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Current revision

Crystal Structure of Human serum albumin complexed with Lidocaine

PDB ID 3jqz

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