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2kt6

From Proteopedia

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{{Seed}}
 
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[[Image:2kt6.jpg|left|200px]]
 
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==Structural homology between the C-terminal domain of the PapC usher and its plug==
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The line below this paragraph, containing "STRUCTURE_2kt6", creates the "Structure Box" on the page.
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<StructureSection load='2kt6' size='340' side='right'caption='[[2kt6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kt6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KT6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3l48|3l48]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">papC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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{{STRUCTURE_2kt6| PDB=2kt6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kt6 OCA], [https://pdbe.org/2kt6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kt6 RCSB], [https://www.ebi.ac.uk/pdbsum/2kt6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kt6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/PAPC_ECOLX PAPC_ECOLX]] Involved in the export and assembly of pili subunits across the outer membrane. Forms a hexameric ring-shaped pore in the outer bacterial membrane. The 2 nanometer-diameter pore allows the passage of the thin tip fibrillum. As for the rod, it probably unwinds into linear fibers which would therefore be narrow enough to pass through the pore.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kt/2kt6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kt6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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P pili are extracellular appendages responsible for the targeting of uropathogenic Escherichia coli to the kidney. They are assembled by the chaperone-usher (CU) pathway of pilus biogenesis involving two proteins, the periplasmic chaperone PapD and the outer membrane assembly platform, PapC. Many aspects of the structural biology of the Pap CU pathway have been elucidated, except for the C-terminal domain of the PapC usher, the structure of which is unknown. In this report, we identify a stable and folded fragment of the C-terminal region of the PapC usher and determine its structure using both X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. These structures reveal a beta-sandwich fold very similar to that of the plug domain, a domain of PapC obstructing its translocation domain. This structural similarity suggests similar functions in usher-mediated pilus biogenesis, playing out at different stages of the process. This structure paves the way for further functional analysis targeting surfaces common to both the plug and the C-terminal domain of PapC.
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===Structural homology between the C-terminal domain of the PapC usher and its plug===
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Structural homology between the C-terminal domain of the PapC usher and its plug.,Ford B, Rego AT, Ragan TJ, Pinkner J, Dodson K, Driscoll PC, Hultgren S, Waksman G J Bacteriol. 2010 Apr;192(7):1824-31. Epub 2010 Jan 29. PMID:20118254<ref>PMID:20118254</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20118254}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2kt6" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20118254 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_20118254}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus coli migula 1895]]
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2KT6 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KT6 OCA].
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[[Category: Large Structures]]
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[[Category: Dodson, K]]
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==Reference==
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[[Category: Driscoll, P C]]
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<ref group="xtra">PMID:20118254</ref><references group="xtra"/>
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[[Category: Ford, B]]
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[[Category: Escherichia coli]]
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[[Category: Hultgren, S]]
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[[Category: Dodson, K.]]
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[[Category: Pinkner, J]]
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[[Category: Driscoll, P C.]]
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[[Category: Ragan, T J]]
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[[Category: Ford, B.]]
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[[Category: Rego, A]]
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[[Category: Hultgren, S.]]
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[[Category: Waksman, G]]
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[[Category: Pinkner, J.]]
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[[Category: Ragan, T J.]]
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[[Category: Rego, A.]]
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[[Category: Waksman, G.]]
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[[Category: Cell membrane]]
[[Category: Cell membrane]]
[[Category: Cell outer membrane]]
[[Category: Cell outer membrane]]
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[[Category: Transport]]
[[Category: Transport]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 21 10:07:12 2010''
 

Current revision

Structural homology between the C-terminal domain of the PapC usher and its plug

PDB ID 2kt6

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