1qni

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[[Image:1qni.jpg|left|200px]]<br /><applet load="1qni" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qni, resolution 2.40&Aring;" />
 
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'''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION'''<br />
 
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==Overview==
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==Crystal Structure of Nitrous Oxide Reductase from Pseudomonas nautica, at 2.4A Resolution==
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Nitrous oxide (N20) is a greenhouse gas, the third most significant, contributor to global warming. As a key process for N20 elimination from, the biosphere, N20 reductases catalyze the two-electron reduction of N20, to N2. These 2 x 65 kDa copper enzymes are thought to contain a CuA, electron entry site, similar to that of cytochrome c oxidase, and a CuZ, catalytic center. The copper anomalous signal was used to solve the, crystal structure of N20 reductase from Pseudomonas nautica by, multiwavelength anomalous dispersion, to a resolution of 2.4 A. The, structure reveals that the CuZ center belongs to a new type of metal, cluster, in which four copper ions are liganded by seven histidine, residues. N20 binds to this center via a single copper ion. The remaining, copper ions might act as an electron reservoir, assuring a fast electron, transfer and avoiding the formation of dead-end products.
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<StructureSection load='1qni' size='340' side='right'caption='[[1qni]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qni]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinobacter_nauticus Marinobacter nauticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QNI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=CUZ:(MU-4-SULFIDO)-TETRA-NUCLEAR+COPPER+ION'>CUZ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qni OCA], [https://pdbe.org/1qni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qni RCSB], [https://www.ebi.ac.uk/pdbsum/1qni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qni ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7SIA3_MARNT Q7SIA3_MARNT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/1qni_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qni ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nitrous oxide (N20) is a greenhouse gas, the third most significant contributor to global warming. As a key process for N20 elimination from the biosphere, N20 reductases catalyze the two-electron reduction of N20 to N2. These 2 x 65 kDa copper enzymes are thought to contain a CuA electron entry site, similar to that of cytochrome c oxidase, and a CuZ catalytic center. The copper anomalous signal was used to solve the crystal structure of N20 reductase from Pseudomonas nautica by multiwavelength anomalous dispersion, to a resolution of 2.4 A. The structure reveals that the CuZ center belongs to a new type of metal cluster, in which four copper ions are liganded by seven histidine residues. N20 binds to this center via a single copper ion. The remaining copper ions might act as an electron reservoir, assuring a fast electron transfer and avoiding the formation of dead-end products.
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==About this Structure==
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A novel type of catalytic copper cluster in nitrous oxide reductase.,Brown K, Tegoni M, Prudencio M, Pereira AS, Besson S, Moura JJ, Moura I, Cambillau C Nat Struct Biol. 2000 Mar;7(3):191-5. PMID:10700275<ref>PMID:10700275</ref>
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1QNI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Marinobacter_hydrocarbonoclasticus Marinobacter hydrocarbonoclasticus] with CA, CL, CUA and CUZ as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=CUA:Cua Center (Electron Entry Gate)'>CUA</scene>, <scene name='pdbsite=CUB:Cua Center (Electron Entry Gate)'>CUB</scene>, <scene name='pdbsite=CUC:Cua Center (Electron Entry Gate)'>CUC</scene>, <scene name='pdbsite=CUD:Cua Center (Electron Entry Gate)'>CUD</scene>, <scene name='pdbsite=CUE:Cua Center (Electron Entry Gate)'>CUE</scene>, <scene name='pdbsite=CUF:Cua Center (Electron Entry Gate)'>CUF</scene>, <scene name='pdbsite=CUU:Cuz Center (Catalytic Site)'>CUU</scene>, <scene name='pdbsite=CUV:Cuz Center (Catalytic Site)'>CUV</scene>, <scene name='pdbsite=CUW:Cuz Center (Catalytic Site)'>CUW</scene>, <scene name='pdbsite=CUX:Cuz Center (Catalytic Site)'>CUX</scene>, <scene name='pdbsite=CUY:Cuz Center (Catalytic Site)'>CUY</scene> and <scene name='pdbsite=CUZ:Cuz Center (Catalytic Site)'>CUZ</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QNI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A novel type of catalytic copper cluster in nitrous oxide reductase., Brown K, Tegoni M, Prudencio M, Pereira AS, Besson S, Moura JJ, Moura I, Cambillau C, Nat Struct Biol. 2000 Mar;7(3):191-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10700275 10700275]
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</div>
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[[Category: Marinobacter hydrocarbonoclasticus]]
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<div class="pdbe-citations 1qni" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Brown, K.]]
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<references/>
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[[Category: Cambillau, C.]]
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__TOC__
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[[Category: Tegoni, M.]]
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</StructureSection>
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[[Category: CA]]
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[[Category: Large Structures]]
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[[Category: CL]]
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[[Category: Marinobacter nauticus]]
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[[Category: CUA]]
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[[Category: Brown K]]
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[[Category: CUZ]]
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[[Category: Cambillau C]]
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[[Category: crystal structure]]
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[[Category: Tegoni M]]
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[[Category: denitrification]]
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[[Category: electron transfer]]
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[[Category: mad]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:57:40 2007''
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Current revision

Crystal Structure of Nitrous Oxide Reductase from Pseudomonas nautica, at 2.4A Resolution

PDB ID 1qni

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