3l7j

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{{Seed}}
 
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[[Image:3l7j.jpg|left|200px]]
 
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==Structure of the Wall Teichoic Acid Polymerase TagF, H444N variant==
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The line below this paragraph, containing "STRUCTURE_3l7j", creates the "Structure Box" on the page.
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<StructureSection load='3l7j' size='340' side='right'caption='[[3l7j]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3l7j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis_RP62A Staphylococcus epidermidis RP62A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L7J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3l7j| PDB=3l7j | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l7j OCA], [https://pdbe.org/3l7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l7j RCSB], [https://www.ebi.ac.uk/pdbsum/3l7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l7j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TAGF_STAEQ TAGF_STAEQ] Responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the disaccharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in length.<ref>PMID:20400947</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l7/3l7j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l7j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Teichoic acid polymers are composed of polyol-phosphate units and form a major component of Gram-positive bacterial cell walls. These anionic compounds perform a multitude of important roles in bacteria and are synthesized by monotopic membrane proteins of the TagF polymerase family. We have determined the structure of Staphylococcus epidermidis TagF to 2.7-A resolution from a construct that includes both the membrane-targeting region and the glycerol-phosphate polymerase domains. TagF possesses a helical region for interaction with the lipid bilayer, placing the active site at a suitable distance for access to the membrane-bound substrate. Characterization of active-site residue variants and analysis of a CDP-glycerol substrate complex suggest a mechanism for polymer synthesis. With the importance of teichoic acid in Gram-positive physiology, this elucidation of the molecular details of TagF function provides a critical new target in the development of novel anti-infectives.
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===Structure of the Wall Teichoic Acid Polymerase TagF, H444N variant===
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Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis.,Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC Nat Struct Mol Biol. 2010 May;17(5):582-9. Epub 2010 Apr 18. PMID:20400947<ref>PMID:20400947</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3L7J is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L7J OCA].
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<div class="pdbe-citations 3l7j" style="background-color:#fffaf0;"></div>
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[[Category: Staphylococcus epidermidis]]
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== References ==
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[[Category: Lovering, A L.]]
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<references/>
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[[Category: Strynadka, N C.J.]]
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__TOC__
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[[Category: Gt-b]]
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</StructureSection>
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[[Category: Monotopic membrane protein]]
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[[Category: Large Structures]]
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[[Category: Structural protein]]
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[[Category: Staphylococcus epidermidis RP62A]]
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[[Category: Lovering AL]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 28 11:07:35 2010''
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[[Category: Strynadka NCJ]]

Current revision

Structure of the Wall Teichoic Acid Polymerase TagF, H444N variant

PDB ID 3l7j

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