3mln
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3mln is ON HOLD Authors: Treiber, N., Treiber, T., Zocher, G., Grosschedl, R. Description: DNA binding domain of Early B-cell Factor 1 (Ebf1) bound...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==DNA binding domain of Early B-cell Factor 1 (Ebf1) bound to DNA (crystal form II)== | |
| + | <StructureSection load='3mln' size='340' side='right'caption='[[3mln]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3mln]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MLN FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mln OCA], [https://pdbe.org/3mln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mln RCSB], [https://www.ebi.ac.uk/pdbsum/3mln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mln ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/COE1_MOUSE COE1_MOUSE] Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.<ref>PMID:20876732</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Early B-cell factor 1 (Ebf1) is a key transcriptional determinant of B-lymphocyte differentiation whose DNA-binding domain has no sequence similarity to other transcription factor families. Here we report the crystal structure of an Ebf1 dimer bound to its palindromic recognition site. The DNA-binding domain adopts a pseudoimmunoglobulin-like fold with novel topology, but is structurally similar to the Rel homology domains of NFAT and NF-kappaB. Ebf1 contacts the DNA with two loop-based modules and a unique Zn coordination motif whereby each Ebf1 monomer interacts with both palindromic half-sites. This unusual mode of DNA recognition generates an extended contact area that may be crucial for the function of Ebf1 in chromatin. | ||
| - | + | Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins.,Treiber N, Treiber T, Zocher G, Grosschedl R Genes Dev. 2010 Oct 15;24(20):2270-5. Epub 2010 Sep 28. PMID:20876732<ref>PMID:20876732</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3mln" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Grosschedl R]] | ||
| + | [[Category: Treiber N]] | ||
| + | [[Category: Treiber T]] | ||
| + | [[Category: Zocher G]] | ||
Current revision
DNA binding domain of Early B-cell Factor 1 (Ebf1) bound to DNA (crystal form II)
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