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1ae7

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[[Image:1ae7.gif|left|200px]]<br />
 
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<applet load="1ae7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ae7, resolution 2.0&Aring;" />
 
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'''NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2'''<br />
 
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==Overview==
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==NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2==
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The three-dimensional structure of notexin has been solved by molecular, replacement methods. The structure has been refined at 2.0 A resolution to, a crystallographic R-value of 16.5% with good stereo-chemistry. The core, of the protein is very similar to other phospholipase A2s (PLA2 s) but, several parts of the molecule are distinctly different. The most, significant differences from PLA2 s from bovine pancreas and rattlesnake, occur in the stretches 56-80 and 85-89. Residue 69, which has been shown, to be important for phospholipase binding, has a different conformation, and different interactions than in other known PLA2s. The C alpha, positions for residues 86-88 differ by about 6 A from both the bovine and, the rattlesnake enzyme. The crystals contain no Ca2+ ions. Instead, a, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?1568473 (full description)]]
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<StructureSection load='1ae7' size='340' side='right'caption='[[1ae7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ae7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Notechis_scutatus_scutatus Notechis scutatus scutatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae7 OCA], [https://pdbe.org/1ae7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA2B_NOTSC PA2B_NOTSC] Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. Is directly toxic to skeletal muscle upon local application in vivo (dystrophic effect). Also has direct nephrotoxicity in experimental mice; a single subcutaneous dose (1.38 ug/kg) produces renal tubular and glomerular damage within 24 hours (PubMed:7580101). PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AE7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Notechis_scutatus_scutatus Notechis scutatus scutatus]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]]. Structure known Active Sites: CAL and CNR. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AE7 OCA]].
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The three-dimensional structure of notexin, a presynaptic neurotoxic phospholipase A2 at 2.0 A resolution., Westerlund B, Nordlund P, Uhlin U, Eaker D, Eklund H, FEBS Lett. 1992 Apr 20;301(2):159-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1568473 1568473]
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[[Category: Large Structures]]
[[Category: Notechis scutatus scutatus]]
[[Category: Notechis scutatus scutatus]]
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[[Category: Phospholipase A(2)]]
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[[Category: Eaker D]]
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[[Category: Single protein]]
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[[Category: Eklund H]]
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[[Category: Eaker, D.]]
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[[Category: Nordlund P]]
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[[Category: Eklund, H.]]
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[[Category: Uhlin U]]
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[[Category: Nordlund, P.]]
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[[Category: Westerlund B]]
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[[Category: Uhlin, U.]]
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[[Category: Westerlund, B.]]
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[[Category: SO4]]
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[[Category: hydrolase]]
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[[Category: lipid degradation]]
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[[Category: phospholipase a2]]
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[[Category: presynaptic neurotoxin]]
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[[Category: venom]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:38:24 2007''
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Current revision

NOTEXIN, A PRESYNAPTIC NEUROTOXIC PHOSPHOLIPASE A2

PDB ID 1ae7

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