1ux7

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[[Image:1ux7.gif|left|200px]]<br /><applet load="1ux7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ux7, resolution 1.50&Aring;" />
 
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'''CARBOHYDRATE-BINDING MODULE CBM36 IN COMPLEX WITH CALCIUM AND XYLOTRIOSE'''<br />
 
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==Overview==
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==Carbohydrate-Binding Module CBM36 in complex with calcium and xylotriose==
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The enzymatic degradation of polysaccharides harnesses multimodular, enzymes whose carbohydrate binding modules (CBM) target the catalytic, domain onto the recalcitrant substrate. Here we report the ab initio, structure determination and subsequent refinement, at 0.8 A resolution, of, the CBM36 domain of the Paenibacillus polymyxa xylanase 43A. Affinity, electrophoresis, isothermal titration calorimetry, and UV difference, spectroscopy demonstrate that CBM36 is a novel Ca(2+)-dependent xylan, binding domain. The 3D structure of CBM36 in complex with xylotriose and, Ca(2+), at 1.5 A resolution, displays significant conformational changes, compared to the native structure and reveals the molecular basis for its, unique Ca(2+)-dependent binding of xylooligosaccharides through, coordination of the O2 and O3 hydroxyls. CBM36 is one of an emerging, spectrum of carbohydrate binding modules that increasingly find, applications in industry and display great potential for mapping the, "glyco-architecture" of plant cells.
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<StructureSection load='1ux7' size='340' side='right'caption='[[1ux7]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ux7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UX7 FirstGlance]. <br>
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1UX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa] with CA and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UX7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ux7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ux7 OCA], [https://pdbe.org/1ux7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ux7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ux7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ux7 ProSAT]</span></td></tr>
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Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242594 15242594]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XYND_PAEPO XYND_PAEPO] Cleaves arabinose units from O-2- or O-3-monosubstituted xylose residues, thereby assisting in arabinoxylan (AX) and short-chain arabinoxylo-oligosaccharide (AXOS) degradation (By similarity). Preferres wheat flour xylan over oat spelt xylan as substrate. Does not display endoxylanase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1ux7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ux7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Paenibacillus polymyxa]]
[[Category: Paenibacillus polymyxa]]
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[[Category: Single protein]]
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[[Category: Boraston AB]]
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[[Category: Boraston, A.B.]]
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[[Category: Davies GJ]]
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[[Category: Davies, G.J.]]
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[[Category: Jamal S]]
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[[Category: Jamal, S.]]
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[[Category: CA]]
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[[Category: SO4]]
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[[Category: calcium]]
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[[Category: carbohydrate binding domain]]
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[[Category: carbohydrate-binding module cbm36]]
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[[Category: hydrolase]]
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[[Category: xylan]]
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[[Category: xylanase gh43]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:15:45 2007''
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Current revision

Carbohydrate-Binding Module CBM36 in complex with calcium and xylotriose

PDB ID 1ux7

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