3iyl

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{{Seed}}
 
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[[Image:3iyl.jpg|left|200px]]
 
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==Atomic CryoEM Structure of a Nonenveloped Virus Suggests How Membrane Penetration Protein is Primed for Cell Entry==
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The line below this paragraph, containing "STRUCTURE_3iyl", creates the "Structure Box" on the page.
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<SX load='3iyl' size='340' side='right' viewer='molstar' caption='[[3iyl]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3iyl]] is a 25 chain structure with sequence from [https://en.wikipedia.org/wiki/Grass_carp_reovirus Grass carp reovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IYL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
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{{STRUCTURE_3iyl| PDB=3iyl | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iyl OCA], [https://pdbe.org/3iyl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iyl RCSB], [https://www.ebi.ac.uk/pdbsum/3iyl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iyl ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iy/3iyl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iyl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To achieve cell entry, many nonenveloped viruses must transform from a dormant to a primed state. In contrast to the membrane fusion mechanism of enveloped viruses (e.g., influenza virus), this membrane penetration mechanism is poorly understood. Here, using single-particle cryo-electron microscopy, we report a 3.3 A structure of the primed, infectious subvirion particle of aquareovirus. The density map reveals side-chain densities of all types of amino acids (except glycine), enabling construction of a full-atom model of the viral particle. Our structure and biochemical results show that priming involves autocleavage of the membrane penetration protein and suggest that Lys84 and Glu76 may facilitate this autocleavage in a nucleophilic attack. We observe a myristoyl group, covalently linked to the N terminus of the penetration protein and embedded in a hydrophobic pocket. These results suggest a well-orchestrated process of nonenveloped virus entry involving autocleavage of the penetration protein prior to exposure of its membrane-insertion finger.
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===Atomic CryoEM Structure of a Nonenveloped Virus Suggests How Membrane Penetration Protein is Primed for Cell Entry===
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3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry.,Zhang X, Jin L, Fang Q, Hui WH, Zhou ZH Cell. 2010 Apr 30;141(3):472-82. Epub 2010 Apr 15. PMID:20398923<ref>PMID:20398923</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3iyl" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20398923}}, adds the Publication Abstract to the page
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20398923 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20398923}}
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__TOC__
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</SX>
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==About this Structure==
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3IYL is a 25 chains structure with sequences from [http://en.wikipedia.org/wiki/Grass_carp_reovirus Grass carp reovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYL OCA].
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==Reference==
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<ref group="xtra">PMID:20398923</ref><references group="xtra"/>
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[[Category: Grass carp reovirus]]
[[Category: Grass carp reovirus]]
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[[Category: Fang, Q.]]
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[[Category: Large Structures]]
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[[Category: Hui, W.]]
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[[Category: Fang Q]]
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[[Category: Jin, L.]]
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[[Category: Hui W]]
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[[Category: Zhang, X.]]
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[[Category: Jin L]]
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[[Category: Zhou, Z H.]]
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[[Category: Zhang X]]
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[[Category: Autocleavage]]
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[[Category: Zhou ZH]]
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[[Category: Icosahedral virus]]
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[[Category: Membrane penetration protein]]
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[[Category: Myristol group]]
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[[Category: Non-enveloped virus]]
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[[Category: Virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 10:59:13 2010''
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Current revision

Atomic CryoEM Structure of a Nonenveloped Virus Suggests How Membrane Penetration Protein is Primed for Cell Entry

3iyl, resolution 3.30Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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