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3m22
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:3m22.jpg|left|200px]] | ||
| - | < | + | ==Crystal structure of TagRFP fluorescent protein== |
| - | + | <StructureSection load='3m22' size='340' side='right'caption='[[3m22]], [[Resolution|resolution]] 2.20Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[3m22]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M22 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | |
| - | -- | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m24|3m24]]</td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m22 OCA], [http://pdbe.org/3m22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m22 RCSB], [http://www.ebi.ac.uk/pdbsum/3m22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m22 ProSAT]</span></td></tr> | |
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m2/3m22_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m22 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We determined the 2.2 A crystal structures of the red fluorescent protein TagRFP and its derivative, the blue fluorescent protein mTagBFP. The crystallographic analysis is consistent with a model in which TagRFP has the trans coplanar anionic chromophore with the conjugated pi-electron system, similar to that of DsRed-like chromophores. Refined conformation of mTagBFP suggests the presence of an N-acylimine functionality in its chromophore and single C(alpha)-C(beta) bond in the Tyr64 side chain. Mass spectrum of mTagBFP chromophore-bearing peptide indicates a loss of 20 Da upon maturation, whereas tandem mass spectrometry reveals that the C(alpha)-N bond in Leu63 is oxidized. These data indicate that mTagBFP has a new type of the chromophore, N-[(5-hydroxy-1H-imidazole-2-yl)methylidene]acetamide. We propose a chemical mechanism in which the DsRed-like chromophore is formed via the mTagBFP-like blue intermediate. | ||
| - | + | Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins.,Subach OM, Malashkevich VN, Zencheck WD, Morozova KS, Piatkevich KD, Almo SC, Verkhusha VV Chem Biol. 2010 Apr 23;17(4):333-41. PMID:20416505<ref>PMID:20416505</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | == | + | </div> |
| - | + | <div class="pdbe-citations 3m22" style="background-color:#fffaf0;"></div> | |
| - | [[Category: Synthetic construct]] | + | == References == |
| - | [[Category: Almo, S C | + | <references/> |
| - | [[Category: Malashkevich, V N | + | __TOC__ |
| - | [[Category: Ramagopal, U A | + | </StructureSection> |
| - | [[Category: Subach, O M | + | [[Category: Large Structures]] |
| - | [[Category: Verkhusha, V V | + | [[Category: Synthetic construct sequences]] |
| + | [[Category: Almo, S C]] | ||
| + | [[Category: Malashkevich, V N]] | ||
| + | [[Category: Ramagopal, U A]] | ||
| + | [[Category: Subach, O M]] | ||
| + | [[Category: Verkhusha, V V]] | ||
[[Category: Acylimine-containing blue and red chromophore]] | [[Category: Acylimine-containing blue and red chromophore]] | ||
[[Category: De novo protein]] | [[Category: De novo protein]] | ||
[[Category: Fluorescent protein]] | [[Category: Fluorescent protein]] | ||
[[Category: Tagrfp]] | [[Category: Tagrfp]] | ||
| - | |||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 12 11:20:58 2010'' | ||
Current revision
Crystal structure of TagRFP fluorescent protein
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