3igo

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920

Structural highlights

3igo is a 1 chain structure with sequence from Cryptosporidium parvum Iowa II. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.25Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A3FQ16_CRYPI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Calcium-dependent protein kinases (CDPKs) have pivotal roles in the calcium-signaling pathway in plants, ciliates and apicomplexan parasites and comprise a calmodulin-dependent kinase (CaMK)-like kinase domain regulated by a calcium-binding domain in the C terminus. To understand this intramolecular mechanism of activation, we solved the structures of the autoinhibited (apo) and activated (calcium-bound) conformations of CDPKs from the apicomplexan parasites Toxoplasma gondii and Cryptosporidium parvum. In the apo form, the C-terminal CDPK activation domain (CAD) resembles a calmodulin protein with an unexpected long helix in the N terminus that inhibits the kinase domain in the same manner as CaMKII. Calcium binding triggers the reorganization of the CAD into a highly intricate fold, leading to its relocation around the base of the kinase domain to a site remote from the substrate binding site. This large conformational change constitutes a distinct mechanism in calcium signal-transduction pathways.

Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.,Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R. Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium. Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473 doi:10.1038/nsmb.1795

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3igo"

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:3igo.png|left|200px]]
-<!--
+==Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920==
-The line below this paragraph, containing "STRUCTURE_3igo", creates the "Structure Box" on the page.
+<StructureSection load='3igo' size='340' side='right'caption='[[3igo]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3igo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum_Iowa_II Cryptosporidium parvum Iowa II]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGO FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
-{{STRUCTURE_3igo|  PDB=3igo  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3igo OCA], [https://pdbe.org/3igo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3igo RCSB], [https://www.ebi.ac.uk/pdbsum/3igo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3igo ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A3FQ16_CRYPI A3FQ16_CRYPI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/3igo_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3igo ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Calcium-dependent protein kinases (CDPKs) have pivotal roles in the calcium-signaling pathway in plants, ciliates and apicomplexan parasites and comprise a calmodulin-dependent kinase (CaMK)-like kinase domain regulated by a calcium-binding domain in the C terminus. To understand this intramolecular mechanism of activation, we solved the structures of the autoinhibited (apo) and activated (calcium-bound) conformations of CDPKs from the apicomplexan parasites Toxoplasma gondii and Cryptosporidium parvum. In the apo form, the C-terminal CDPK activation domain (CAD) resembles a calmodulin protein with an unexpected long helix in the N terminus that inhibits the kinase domain in the same manner as CaMKII. Calcium binding triggers the reorganization of the CAD into a highly intricate fold, leading to its relocation around the base of the kinase domain to a site remote from the substrate binding site. This large conformational change constitutes a distinct mechanism in calcium signal-transduction pathways.
-===Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920===
+Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.,Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473<ref>PMID:20436473</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3igo" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20436473}}, adds the Publication Abstract to the page
+*[[Calcium-dependent protein kinase|Calcium-dependent protein kinase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20436473 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20436473}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Cryptosporidium parvum Iowa II]]
-IGO is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum_iowa_ii Cryptosporidium parvum iowa ii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGO OCA].
+[[Category: Large Structures]]
+[[Category: Allali-Hassanali A]]
-==Reference==
+[[Category: Amani M]]
-<ref group="xtra">PMID:20436473</ref><references group="xtra"/>
+[[Category: Arrowsmith CH]]
-[[Category: Cryptosporidium parvum iowa ii]]
+[[Category: Artz JD]]
-[[Category: Allali-Hassanali, A.]]
+[[Category: Bochkarev A]]
-[[Category: Amani, M.]]
+[[Category: Bountra C]]
-[[Category: Arrowsmith, C H.]]
+[[Category: Edwards AM]]
-[[Category: Artz, J D.]]
+[[Category: Finnerty P]]
-[[Category: Bochkarev, A.]]
+[[Category: Hui R]]
-[[Category: Bountra, C.]]
+[[Category: Lin YH]]
-[[Category: Edwards, A M.]]
+[[Category: MacKenzie F]]
-[[Category: Finnerty, P.]]
+[[Category: Tempel W]]
-[[Category: Hui, R.]]
+[[Category: Vedadi M]]
-[[Category: Lin, Y H.]]
+[[Category: Weigelt J]]
-[[Category: MacKenzie, F.]]
+[[Category: Wernimont AK]]
-[[Category: SGC, Structural Genomics Consortium.]]
-[[Category: Tempel, W.]]
-[[Category: Vedadi, M.]]
-[[Category: Weigelt, J.]]
-[[Category: Wernimont, A K.]]
-[[Category: Atp-binding]]
-[[Category: Calcium]]
-[[Category: Cdpk]]
-[[Category: Kinase]]
-[[Category: Nucleotide-binding]]
-[[Category: Parasite]]
-[[Category: Serine/threonine-protein kinase]]
-[[Category: Sgc]]
-[[Category: Structural genomic]]
-[[Category: Structural genomics consortium]]
-[[Category: Transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 20 09:01:07 2010''

3igo

From Proteopedia

Current revision

Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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