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3myo
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3myo is ON HOLD Authors: Lee, S.J., Kim, H.S., Kim, D.J., Yoon, H.J., Kim, K.H., Yoon, J.Y., Suh, S.W. Description: Crystal structure of tagatose-1...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes== | |
| + | <StructureSection load='3myo' size='340' side='right'caption='[[3myo]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3myo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MYO FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3myo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3myo OCA], [https://pdbe.org/3myo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3myo RCSB], [https://www.ebi.ac.uk/pdbsum/3myo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3myo ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LACD1_STRP1 LACD1_STRP1] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Staphylococcus aureus LacD, a Class I tagatose-1,6-bisphosphate (TBP) aldolase, shows broadened substrate specificity by catalyzing the cleavage of 1,6-bisphosphate derivatives of d-tagatose, d-fructose, d-sorbose, and d-psicose. LacD.1 and LacD.2 are two closely-related Class I TBP aldolases in Streptococcus pyogenes. Here we have determined the crystal structures of S. aureus LacD and S. pyogenes LacD.1. Monomers of both enzymes are folded into a (beta/alpha)(8) barrel and two monomers associate tightly to form a dimer in the crystals. The structures suggest that the residues E189 and S300 of rabbit muscle Class I fructose-1,6-bisphosphate (FBP) aldolase are important for substrate specificity. When we mutated the corresponding residues of S. aureus LacD, the mutants (L165E, L275S, and L165E/L275S) showed enhanced substrate specificity toward FBP. STRUCTURED SUMMARY: lacDbinds to lacD by X-ray crystallography(View interaction) lacD1binds to lacD1 by X-ray crystallography(View interaction). | ||
| - | + | Crystal structures of LacD from Staphylococcus aureus and LacD.1 from Streptococcus pyogenes: Insights into substrate specificity and virulence gene regulation.,Lee SJ, Kim HS, Kim do J, Yoon HJ, Kim KH, Yoon JY, Suh SW FEBS Lett. 2011 Jan 21;585(2):307-12. Epub 2010 Dec 28. PMID:21192932<ref>PMID:21192932</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3myo" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| + | *[[Aldolase 3D structures|Aldolase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptococcus pyogenes serotype M1]] | ||
| + | [[Category: Kim DJ]] | ||
| + | [[Category: Kim HS]] | ||
| + | [[Category: Kim KH]] | ||
| + | [[Category: Lee SJ]] | ||
| + | [[Category: Suh SW]] | ||
| + | [[Category: Yoon HJ]] | ||
| + | [[Category: Yoon JY]] | ||
Current revision
Crystal structure of tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes
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Categories: Large Structures | Streptococcus pyogenes serotype M1 | Kim DJ | Kim HS | Kim KH | Lee SJ | Suh SW | Yoon HJ | Yoon JY
