2xf1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2xf1 is ON HOLD until Paper Publication Authors: Singh, B.K., Sattler, J.M., Huttu, J., Chatterjee, M., Schueler, H., Kursula, I. Description: Crys...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Plasmodium falciparum actin depolymerization factor 1== | |
| + | <StructureSection load='2xf1' size='340' side='right'caption='[[2xf1]], [[Resolution|resolution]] 1.96Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2xf1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XF1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xf1 OCA], [https://pdbe.org/2xf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xf1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xf1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CADF1_PLAF7 CADF1_PLAF7] Not involved in actin polymerisation, instead functions to stimulate nucleotide exchange on monomeric actin and influence turnover of the small amount of cytosolic actin microfilaments. Essential for erythrocytic schizogony (By similarity).[UniProtKB:P86292] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Apicomplexan parasites, such as the malaria-causing Plasmodium, utilize an actin-based motor for motility and host cell invasion. The actin filaments of these parasites are unusually short, and actin polymerization is under strict control of a small set of regulatory proteins, which are poorly conserved with their mammalian orthologs. Actin depolymerization factors (ADFs) are among the most important actin regulators, affecting the rates of filament turnover in a multifaceted manner. Plasmodium has two ADFs that display low sequence homology with each other and with the higher eukaryotic family members. Here, we show that ADF2, like canonical ADF proteins but unlike ADF1, binds to both globular and filamentous actin, severing filaments and inducing nucleotide exchange on the actin monomer. The crystal structure of Plasmodium ADF1 shows major differences from the ADF consensus, explaining the lack of F-actin binding. Plasmodium ADF2 structurally resembles the canonical members of the ADF/cofilin family. | ||
| - | + | Crystal structures explain functional differences in the two actin depolymerization factors of the malaria parasite.,Singh BK, Sattler JM, Chatterjee M, Huttu J, Schuler H, Kursula I J Biol Chem. 2011 Aug 12;286(32):28256-64. PMID:21832095<ref>PMID:21832095</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2xf1" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Plasmodium falciparum]] | ||
| + | [[Category: Chatterjee M]] | ||
| + | [[Category: Huttu J]] | ||
| + | [[Category: Kursula I]] | ||
| + | [[Category: Sattler JM]] | ||
| + | [[Category: Schueler H]] | ||
| + | [[Category: Singh BK]] | ||
Current revision
Crystal structure of Plasmodium falciparum actin depolymerization factor 1
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