3n1x

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(New page: '''Unreleased structure''' The entry 3n1x is ON HOLD Authors: Sazinsky, M.H., McCormick, M.S., Lippard, S.J. Description: X-ray Crystal Structure of Toluene/o-Xylene Monooxygenase Hydr...)
Current revision (09:06, 6 September 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 3n1x is ON HOLD
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==X-ray Crystal Structure of Toluene/o-Xylene Monooxygenase Hydroxylase T201C Mutant==
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<StructureSection load='3n1x' size='340' side='right'caption='[[3n1x]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3n1x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._OX1 Pseudomonas sp. OX1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N1X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n1x OCA], [https://pdbe.org/3n1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n1x RCSB], [https://www.ebi.ac.uk/pdbsum/3n1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n1x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6IV66_9PSED Q6IV66_9PSED]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Toluene/o-xylene monooxygenase hydroxylase (ToMOH), a diiron-containing enzyme, can activate dioxygen to oxidize aromatic substrates. To elucidate the role of a strictly conserved T201 residue during dioxygen activation of the enzyme, T201S, T201G, T201C, and T201V variants of ToMOH were prepared by site-directed mutagenesis. X-ray crystal structures of all the variants were obtained. Steady-state activity, regiospecificity, and single-turnover yields were also determined for the T201 mutants. Dioxygen activation by the reduced T201 variants was explored by stopped-flow UV-vis and Mossbauer spectroscopy. These studies demonstrate that the dioxygen activation mechanism is preserved in all T201 variants; however, both the formation and decay kinetics of a peroxodiiron(III) intermediate, T201(peroxo), were greatly altered, revealing that T201 is critically involved in dioxygen activation. A comparison of the kinetics of O(2) activation in the T201S, T201C, and T201G variants under various reaction conditions revealed that T201 plays a major role in proton transfer, which is required to generate the peroxodiiron(III) intermediate. A mechanism is postulated for dioxygen activation, and possible structures of oxygenated intermediates are discussed.
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Authors: Sazinsky, M.H., McCormick, M.S., Lippard, S.J.
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Active site threonine facilitates proton transfer during dioxygen activation at the diiron center of toluene/o-xylene monooxygenase hydroxylase.,Song WJ, McCormick MS, Behan RK, Sazinsky MH, Jiang W, Lin J, Krebs C, Lippard SJ J Am Chem Soc. 2010 Oct 6;132(39):13582-5. PMID:20839885<ref>PMID:20839885</ref>
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Description: X-ray Crystal Structure of Toluene/o-Xylene Monooxygenase Hydroxylase T201C Mutant
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n1x" style="background-color:#fffaf0;"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 26 08:23:15 2010''
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==See Also==
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*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas sp. OX1]]
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[[Category: Lippard SJ]]
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[[Category: McCormick MS]]
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[[Category: Sazinsky MH]]

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X-ray Crystal Structure of Toluene/o-Xylene Monooxygenase Hydroxylase T201C Mutant

PDB ID 3n1x

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