2wf6

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{{Seed}}
 
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[[Image:2wf6.jpg|left|200px]]
 
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==Structure of Beta-Phosphoglucomutase inhibited with Glucose-6-phosphate and Aluminium tetrafluoride==
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The line below this paragraph, containing "STRUCTURE_2wf6", creates the "Structure Box" on the page.
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<StructureSection load='2wf6' size='340' side='right'caption='[[2wf6]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wf6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WF6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WF6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=BG6:BETA-D-GLUCOSE-6-PHOSPHATE'>BG6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2wf6| PDB=2wf6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wf6 OCA], [https://pdbe.org/2wf6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wf6 RCSB], [https://www.ebi.ac.uk/pdbsum/2wf6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wf6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wf/2wf6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wf6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Prior evidence supporting the direct observation of phosphorane intermediates in enzymatic phosphoryl transfer reactions was based on the interpretation of electron density corresponding to trigonal species bridging the donor and acceptor atoms. Close examination of the crystalline state of beta-phosphoglucomutase, the archetypal phosphorane intermediate-containing enzyme, reveals that the trigonal species is not , but is (trifluoromagnesate). Although complexes are transition state analogues rather than phosphoryl group transfer reaction intermediates, the presence of fluorine nuclei in near-transition state conformations offers new opportunities to explore the nature of the interactions, in particular the independent measures of local electrostatic and hydrogen-bonding distributions using NMR. Measurements on three -sugar phosphate complexes show a remarkable relationship between NMR chemical shifts, primary isotope shifts, NOEs, cross hydrogen bond scalar couplings, and the atomic positions determined from the high-resolution crystal structure of the complex. The measurements provide independent validation of the structural and isoelectronic model of near-transition state conformations.
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===STRUCTURE OF BETA-PHOSPHOGLUCOMUTASE INHIBITED WITH GLUCOSE-6-PHOSPAHTE AND ALUMINIUM TETRAFLUORIDE===
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Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by Formula rather than by phosphoranes.,Baxter NJ, Bowler MW, Alizadeh T, Cliff MJ, Hounslow AM, Wu B, Berkowitz DB, Williams NH, Blackburn GM, Waltho JP Proc Natl Acad Sci U S A. 2010 Feb 24. PMID:20164409<ref>PMID:20164409</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wf6" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2WF6 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WF6 OCA].
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*[[Beta-phosphoglucomutase 3D structures|Beta-phosphoglucomutase 3D structures]]
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[[Category: Beta-phosphoglucomutase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
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[[Category: Alizadeh, T.]]
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[[Category: Large Structures]]
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[[Category: Baxter, N J.]]
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[[Category: Alizadeh T]]
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[[Category: Bermel, W.]]
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[[Category: Baxter NJ]]
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[[Category: Blackburn, G M.]]
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[[Category: Bermel W]]
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[[Category: Bowler, M W.]]
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[[Category: Blackburn GM]]
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[[Category: Cliff, M J.]]
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[[Category: Bowler MW]]
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[[Category: Hollfelder, F.]]
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[[Category: Cliff MJ]]
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[[Category: Hounslow, A M.]]
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[[Category: Hollfelder F]]
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[[Category: Pollard, S.]]
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[[Category: Hounslow AM]]
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[[Category: Waltho, J P.]]
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[[Category: Pollard S]]
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[[Category: Webster, C E.]]
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[[Category: Waltho JP]]
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[[Category: Williams, N H.]]
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[[Category: Webster CE]]
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[[Category: Haloacid dehalogenase superfamily]]
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[[Category: Williams NH]]
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[[Category: Isomerase]]
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[[Category: Phosphotransferase]]
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[[Category: Transition state analogue]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 26 08:27:58 2010''
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Current revision

Structure of Beta-Phosphoglucomutase inhibited with Glucose-6-phosphate and Aluminium tetrafluoride

PDB ID 2wf6

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