3lwi

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{{Seed}}
 
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[[Image:3lwi.jpg|left|200px]]
 
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==Crystal structure of Cren7-dsDNA complex==
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The line below this paragraph, containing "STRUCTURE_3lwi", creates the "Structure Box" on the page.
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<StructureSection load='3lwi' size='340' side='right'caption='[[3lwi]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3lwi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LWI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lwi OCA], [https://pdbe.org/3lwi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lwi RCSB], [https://www.ebi.ac.uk/pdbsum/3lwi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lwi ProSAT]</span></td></tr>
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{{STRUCTURE_3lwi| PDB=3lwi | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CREN7_SACS2 CREN7_SACS2] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/3lwi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lwi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cren7, a newly found chromatin protein, is highly conserved in the Crenarchaeota. The protein shows higher affinity for double-stranded DNA than for single-stranded DNA, constrains negative DNA supercoils in vitro and is associated with genomic DNA in vivo. Here we report the crystal structures of the Cren7 protein from Sulfolobus solfataricus in complex with two DNA sequences. Cren7 binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 53 degrees) through the intercalation of the hydrophobic side chain of Leu28. Loop beta 3-beta 4 of Cren7 undergoes a significant conformational change upon binding of the protein to DNA, suggesting its critical role in the stabilization of the protein-DNA complex. The roles of DNA-contacting amino acid residues in stabilizing the Cren7-DNA interaction were examined by mutational analysis. Structural comparison of Cren7-DNA complexes with Sac7d-DNA complexes reveals significant differences between the two proteins in DNA binding surface, suggesting that Cren7 and Sul7d serve distinct functions in chromosomal organization.
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===Crystal structure of Cren7-dsDNA complex===
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Structural insights into the interaction of the crenarchaeal chromatin protein Cren7 with DNA.,Zhang Z, Gong Y, Guo L, Jiang T, Huang L Mol Microbiol. 2010 May;76(3):749-59. Epub 2010 Mar 16. PMID:20345658<ref>PMID:20345658</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20345658}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3lwi" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20345658 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20345658}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3LWI is a 6 chains structure with sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LWI OCA].
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: Gong Y]]
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==Reference==
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[[Category: Guo L]]
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<ref group="xtra">PMID:20345658</ref><references group="xtra"/>
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[[Category: Huang L]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Jiang T]]
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[[Category: Gong, Y.]]
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[[Category: Zhang ZF]]
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[[Category: Guo, L.]]
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[[Category: Huang, L.]]
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[[Category: Jiang, T.]]
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[[Category: Zhang, Z F.]]
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[[Category: Beta-sheet]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Dna-binding]]
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[[Category: Methylation]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 26 08:33:52 2010''
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Current revision

Crystal structure of Cren7-dsDNA complex

PDB ID 3lwi

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