3m24

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[[Image:3m24.jpg|left|200px]]
 
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==Crystal structure of TagBFP fluorescent protein==
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The line below this paragraph, containing "STRUCTURE_3m24", creates the "Structure Box" on the page.
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<StructureSection load='3m24' size='340' side='right'caption='[[3m24]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3m24]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. The June 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''GFP-like Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_6 10.2210/rcsb_pdb/mom_2014_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M24 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NRP:{4-[(4-HYDROXYPHENYL)METHYLIDENE]-2-[(1E)-3-METHYLBUTANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRP</scene></td></tr>
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{{STRUCTURE_3m24| PDB=3m24 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m24 OCA], [https://pdbe.org/3m24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m24 RCSB], [https://www.ebi.ac.uk/pdbsum/3m24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m24 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m2/3m24_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m24 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We determined the 2.2 A crystal structures of the red fluorescent protein TagRFP and its derivative, the blue fluorescent protein mTagBFP. The crystallographic analysis is consistent with a model in which TagRFP has the trans coplanar anionic chromophore with the conjugated pi-electron system, similar to that of DsRed-like chromophores. Refined conformation of mTagBFP suggests the presence of an N-acylimine functionality in its chromophore and single C(alpha)-C(beta) bond in the Tyr64 side chain. Mass spectrum of mTagBFP chromophore-bearing peptide indicates a loss of 20 Da upon maturation, whereas tandem mass spectrometry reveals that the C(alpha)-N bond in Leu63 is oxidized. These data indicate that mTagBFP has a new type of the chromophore, N-[(5-hydroxy-1H-imidazole-2-yl)methylidene]acetamide. We propose a chemical mechanism in which the DsRed-like chromophore is formed via the mTagBFP-like blue intermediate.
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===Crystal structure of TagBFP fluorescent protein===
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Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins.,Subach OM, Malashkevich VN, Zencheck WD, Morozova KS, Piatkevich KD, Almo SC, Verkhusha VV Chem Biol. 2010 Apr 23;17(4):333-41. PMID:20416505<ref>PMID:20416505</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3m24" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20416505 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20416505}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: GFP-like Proteins]]
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3M24 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M24 OCA].
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
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==Reference==
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<ref group="xtra">PMID:20416505</ref><references group="xtra"/>
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[[Category: Synthetic construct]]
[[Category: Synthetic construct]]
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[[Category: Almo, S C.]]
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[[Category: Almo SC]]
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[[Category: Malashkevich, V N.]]
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[[Category: Malashkevich VN]]
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[[Category: Subach, O M.]]
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[[Category: Subach OM]]
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[[Category: Verkhusha, V V.]]
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[[Category: Verkhusha VV]]
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[[Category: Acylimine-containing blue and red chromophore]]
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[[Category: De novo protein]]
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[[Category: Fluorescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 26 08:34:04 2010''
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Current revision

Crystal structure of TagBFP fluorescent protein

PDB ID 3m24

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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