3i77

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{{Seed}}
 
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[[Image:3i77.jpg|left|200px]]
 
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==35/99/170-loops of FXa in SGT==
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The line below this paragraph, containing "STRUCTURE_3i77", creates the "Structure Box" on the page.
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<StructureSection load='3i77' size='340' side='right'caption='[[3i77]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3i77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I77 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3i77| PDB=3i77 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i77 OCA], [https://pdbe.org/3i77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i77 RCSB], [https://www.ebi.ac.uk/pdbsum/3i77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i77 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRYP_STRGR TRYP_STRGR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/3i77_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i77 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Converting one enzyme into another is challenging due to the uneven distribution of important amino acids for function in both protein sequence and structure. We report a strategy for protein engineering allowing an organized mixing and matching of genetic material that leverages lower throughput with increased quality of screens. Our approach successfully tested the contribution of each surface-exposed loop in the trypsin fold alone and the cooperativity of their combinations towards building the substrate selectivity and Na(+)-dependent allosteric activation of the protease domain of human coagulation factor Xa into a bacterial trypsin. As the created proteases lack additional protein domains and protein co-factor activation mechanism requisite for the complexity of blood coagulation, they are stepping-stones towards further understanding and engineering of artificial clotting factors.
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===35/99/170-loops of FXa in SGT===
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Combinatorial enzyme design probes allostery and cooperativity in the trypsin fold.,Page MJ, Di Cera E J Mol Biol. 2010 Jun 4;399(2):306-19. Epub 2010 Apr 24. PMID:20399789<ref>PMID:20399789</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3i77" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20399789}}, adds the Publication Abstract to the page
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20399789 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20399789}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3I77 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I77 OCA].
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==Reference==
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<ref group="xtra">PMID:20399789</ref><references group="xtra"/>
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[[Category: Streptomyces griseus]]
[[Category: Streptomyces griseus]]
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[[Category: Trypsin]]
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[[Category: Di Cera E]]
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[[Category: Cera, E Di.]]
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[[Category: Page MJ]]
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[[Category: Page, M J.]]
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[[Category: Beta sheet]]
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[[Category: Disulfide bond]]
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[[Category: Hydrolase]]
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[[Category: Protease]]
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[[Category: Serine protease]]
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[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 2 08:36:06 2010''
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Current revision

35/99/170-loops of FXa in SGT

PDB ID 3i77

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