2bhr

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[[Image:2bhr.gif|left|200px]]<br /><applet load="2bhr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bhr, resolution 2.8&Aring;" />
 
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'''DENGUE VIRUS RNA HELICASE'''<br />
 
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==Overview==
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==Dengue virus RNA helicase==
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Dengue fever is an important emerging public health concern, with several, million viral infections occurring annually, for which no effective, therapy currently exists. The NS3 protein from Dengue virus is a, multifunctional protein of 69 kDa, endowed with protease, helicase, and, nucleoside 5'-triphosphatase (NTPase) activities. Thus, NS3 plays an, important role in viral replication and represents a very interesting, target for the development of specific antiviral inhibitors. We present, the structure of an enzymatically active fragment of the Dengue virus, NTPase/helicase catalytic domain to 2.4 A resolution. The structure is, composed of three domains, displays an asymmetric distribution of charges, on its surface, and contains a tunnel large enough to accommodate, single-stranded RNA. Its C-terminal domain adopts a new fold compared to, the NS3 helicase of hepatitis C virus, which has interesting implications, for the evolution of the Flaviviridae replication complex. A bound sulfate, ion reveals residues involved in the metal-dependent NTPase catalytic, mechanism. Comparison with the NS3 hepatitis C virus helicase complexed to, single-stranded DNA would place the 3' single-stranded tail of a nucleic, acid duplex in the tunnel that runs across the basic face of the protein., A possible model for the unwinding mechanism is proposed.
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<StructureSection load='2bhr' size='340' side='right'caption='[[2bhr]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bhr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BHR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bhr OCA], [https://pdbe.org/2bhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bhr RCSB], [https://www.ebi.ac.uk/pdbsum/2bhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bhr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q91H74_9FLAV Q91H74_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/2bhr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bhr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Dengue fever is an important emerging public health concern, with several million viral infections occurring annually, for which no effective therapy currently exists. The NS3 protein from Dengue virus is a multifunctional protein of 69 kDa, endowed with protease, helicase, and nucleoside 5'-triphosphatase (NTPase) activities. Thus, NS3 plays an important role in viral replication and represents a very interesting target for the development of specific antiviral inhibitors. We present the structure of an enzymatically active fragment of the Dengue virus NTPase/helicase catalytic domain to 2.4 A resolution. The structure is composed of three domains, displays an asymmetric distribution of charges on its surface, and contains a tunnel large enough to accommodate single-stranded RNA. Its C-terminal domain adopts a new fold compared to the NS3 helicase of hepatitis C virus, which has interesting implications for the evolution of the Flaviviridae replication complex. A bound sulfate ion reveals residues involved in the metal-dependent NTPase catalytic mechanism. Comparison with the NS3 hepatitis C virus helicase complexed to single-stranded DNA would place the 3' single-stranded tail of a nucleic acid duplex in the tunnel that runs across the basic face of the protein. A possible model for the unwinding mechanism is proposed.
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==About this Structure==
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Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A.,Xu T, Sampath A, Chao A, Wen D, Nanao M, Chene P, Vasudevan SG, Lescar J J Virol. 2005 Aug;79(16):10278-88. PMID:16051821<ref>PMID:16051821</ref>
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2BHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHR OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A., Xu T, Sampath A, Chao A, Wen D, Nanao M, Chene P, Vasudevan SG, Lescar J, J Virol. 2005 Aug;79(16):10278-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16051821 16051821]
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</div>
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[[Category: Dengue virus type 3]]
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<div class="pdbe-citations 2bhr" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Chao, A.]]
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[[Category: Chene, P.]]
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[[Category: Lescar, J.]]
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[[Category: Nanao, M.]]
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[[Category: Sampath, A.]]
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[[Category: Vasudevan, S.G.]]
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[[Category: Wen, D.]]
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[[Category: Xu, T.]]
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[[Category: SO4]]
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[[Category: dengue virus]]
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[[Category: nucleoside triphosphatase]]
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[[Category: rna helicase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:47:28 2007''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Dengue virus 2]]
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[[Category: Large Structures]]
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[[Category: Chao A]]
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[[Category: Chene P]]
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[[Category: Lescar J]]
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[[Category: Nanao M]]
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[[Category: Sampath A]]
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[[Category: Vasudevan SG]]
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[[Category: Wen D]]
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[[Category: Xu T]]

Current revision

Dengue virus RNA helicase

PDB ID 2bhr

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