3kxt

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{{Seed}}
 
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[[Image:3kxt.png|left|200px]]
 
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==Crystal structure of Sulfolobus Cren7-dsDNA complex==
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The line below this paragraph, containing "STRUCTURE_3kxt", creates the "Structure Box" on the page.
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<StructureSection load='3kxt' size='340' side='right'caption='[[3kxt]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kxt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KXT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.602&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxt OCA], [https://pdbe.org/3kxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kxt RCSB], [https://www.ebi.ac.uk/pdbsum/3kxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kxt ProSAT]</span></td></tr>
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{{STRUCTURE_3kxt| PDB=3kxt | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CREN7_SACS2 CREN7_SACS2] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/3kxt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kxt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cren7 is a crenarchaeal conserved chromatin protein discovered recently. To explore the mechanism of the DNA packaging in Crenarchaeota, the crystal structure of Cren7-GCGATCGC complex has been determined and refined at 1.6 A resolution. Cren7 kinks the dsDNA sharply similar to Sul7d, another chromatin protein existing only in Sulfolobales, which reveals that the "bending and unwinding" compacting mechanism is conserved in Crenarchaeota. Significant structural differences are revealed by comparing both protein-dsDNA complexes. The kinked sites on the same dsDNA in the complexes with Sul7d and Cren7 show one base pair shift. For Cren7, fewer charged residues in the beta-barrel structural region bind to DNA, and additionally, the flexible loop L(beta3beta4) is also involved in the binding. Electrophoretic mobility shift assays indicate that loop L(beta3beta4) is essential for DNA-binding of Cren7. These differences provide insight into the functional difference of both chromatin proteins, suggesting that Cren7 may be more regulative than Sul7d in the DNA-binding affinity by the methylation in the flexible loop L(beta3beta4) in vivo.
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===Crystal structure of Sulfolobus Cren7-dsDNA complex===
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Crystal structure of the crenarchaeal conserved chromatin protein Cren7 and double-stranded DNA complex.,Feng Y, Yao H, Wang J Protein Sci. 2010 Jun;19(6):1253-7. PMID:20512977<ref>PMID:20512977</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20512977}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kxt" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20512977 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20512977}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KXT is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXT OCA].
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[[Category: Saccharolobus solfataricus]]
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[[Category: Feng Y]]
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==Reference==
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[[Category: Wang J]]
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<ref group="xtra">PMID:20512977</ref><references group="xtra"/>
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[[Category: Sulfolobus solfataricus]]
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[[Category: Feng, Y.]]
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[[Category: Wang, J.]]
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[[Category: Crenarchaea chromatin protein]]
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[[Category: Dna binding protein-dna complex]]
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[[Category: Methylation]]
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[[Category: Minor-groove binding]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 16 08:10:22 2010''
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Current revision

Crystal structure of Sulfolobus Cren7-dsDNA complex

PDB ID 3kxt

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