2x3h

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{{Seed}}
 
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[[Image:2x3h.png|left|200px]]
 
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==COLIPHAGE K5A LYASE==
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The line below this paragraph, containing "STRUCTURE_2x3h", creates the "Structure Box" on the page.
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<StructureSection load='2x3h' size='340' side='right'caption='[[2x3h]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x3h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_K1-5 Escherichia virus K1-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
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{{STRUCTURE_2x3h| PDB=2x3h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3h OCA], [https://pdbe.org/2x3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3h RCSB], [https://www.ebi.ac.uk/pdbsum/2x3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O09496_9CAUD O09496_9CAUD]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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K5 lyase A (KflA) is a tail spike protein (TSP) encoded by a K5A coliphage, which cleaves K5 capsular polysaccharide, a glycosaminoglycan with the repeat unit [-4)-betaGlcA-(1,4)- alphaGlcNAc(1-], displayed on the surface of Escherichia coli K5 strains. The crystal structure of KflA reveals a trimeric arrangement, with each monomer containing a right-handed, single-stranded parallel beta-helix domain. Stable trimer formation by the intertwining of strands in the C-terminal domain, followed by proteolytic maturation, is likely to be catalyzed by an autochaperone as described for K1F endosialidase. The structure of KflA represents the first bacteriophage tail spike protein combining polysaccharide lyase activity with a single-stranded parallel beta-helix fold. We propose a catalytic site and mechanism representing convergence with the syn-beta-elimination site of heparinase II from Pedobacter heparinus.
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===COLIPHAGE K5A LYASE===
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The K5 lyase KflA combines a viral tail spike structure with a bacterial polysaccharide lyase mechanism.,Thompson JE, Pourhossein M, Waterhouse A, Hudson T, Goldrick M, Derrick JP, Roberts IS J Biol Chem. 2010 Jul 30;285(31):23963-9. Epub 2010 Jun 2. PMID:20519506<ref>PMID:20519506</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20519506}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x3h" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20519506 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20519506}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus K1-5]]
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2X3H is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3H OCA].
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[[Category: Large Structures]]
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[[Category: Derrick JP]]
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==Reference==
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[[Category: Goldrick M]]
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<ref group="xtra">PMID:20519506</ref><references group="xtra"/>
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[[Category: Hudson T]]
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[[Category: Heparin lyase]]
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[[Category: Pourhossein M]]
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[[Category: Viruses]]
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[[Category: Roberts IS]]
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[[Category: Derrick, J P.]]
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[[Category: Thompson JE]]
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[[Category: Goldrick, M.]]
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[[Category: Hudson, T.]]
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[[Category: Pourhossein, M.]]
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[[Category: Roberts, I S.]]
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[[Category: Thompson, J E.]]
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[[Category: Bacteriophage]]
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[[Category: Glycosaminoglycan]]
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[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 16 08:26:01 2010''
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Current revision

COLIPHAGE K5A LYASE

PDB ID 2x3h

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